Table 1: Oligonucleotides used as primers in this study	
Primer Sequence	Description of use	

ATCCTGGCGCCCCCTCCTGCGGGCACGCATGCGCCTTTGACCTGATGCGGTATTTTCTCC	
1st Transf., Fig 2A, 5'	
GTGCGTATCTTTGTTTACTTGTAGTCTTCGAGGGGAAGGATAACTATGCGGCATCAGAGC	1st Transf., Fig 2A, 3'	
GGTAAAGATACGATAAGAAAAGCCTTTGAACTGCCCATCCTAACTATGCGGCATCAGAGC	1st Transf., Fig 2B, 5'	
CTTCTAGTCCCTTTGCAGACATGCATGACACTTCCTTACCCCTGATGCGGTATTTTCTCC	1st Transf., Fig 2B, 3'	
CGGTAAAACGTTACACTGCCAATGTGTTATAGCAGCC TA ACT ATG CGG CAT CAG AGC	1st Transf., Fig 2C & Fig. 3A i, 5'	
CGCAGTATTGAAGGTACCATCACATGCTTACTCACTTTGCCCTGATGCGGTATTTTCTCC	1st Transf., Fig 2C & Fig. 3A i, 3'	
TGATGATTCACCGGGGAAGAAAGGAATTACAGAACCCCCATAACTATGCGGCATCAGAGC	1st Transf., Fig. 3A ii, 5'	
GTTTCCCGATATAGAAAACTCAGTGGCTGTGTTACGTGGCCCTGATGCGGTATTTTCTCC	1st Transf., Fig. 3A ii, 3'	
GAACTATACAAGCCGCTGGATAAAGCTTATTCGTTGTAACTATGCGGCATCAGAGC	1st Transf., Fig. 3A iii, 5'	
GACTTAAACCATCAAAGGGTGCTCTAGATCTCATC CCTGATGCGGTATTTTCTCC	1st Transf., Fig. 3A iii, 3'	
		
CCGTACAGCTATCGTTTCAGG	5' Diagnostic for URA3 insertion, and primer for error-prone PCR	
AACGTTGTAAACGCAATCACC	3' Diagnostic for URA3 insertion, and primer for error-prone PCR	
		
TGGTACCTTCAATACTGCGTTAGCGAAAAAAAG TGCGTAT	2nd Transf. Fig. 2A, 5'	
GCTCCCTTTAAAATGCACTACG	2nd Transf. Fig. 2A, 3'	
TGGTACCTTCAATACTGCGTTAGC	5' diagnostic (mut.) , Fig. 2A	
TGGTACCTTCAATACTGCGTTGGA	5' diagnostic (WT.) , Fig. 2A	
TCTTGATCGGTGGTTGTGTC	3' diagnostic , Fig. 2A	
		
CAATCTTGAC GATTCACCGGGGAAG	2nd Transf. Fig. 2B, 5'	
 TTTCACCAGTAATGGGTGCCAGTAGG 	2nd Transf. Fig. 2B, 3'	
GCTTAGCCCAATCTTGAC	5' diagnostic (mut.)., Fig. 2B	
GCTTAGCCCAATCTTGAT	5' diagnostic (WT.), Fig. 2B	
AGAGGATCTGGTACTTTCAGAGC	3' diagnostic, Fig. 2B	
		
GTGCGTATCTTTGTTTACTTGTAGTCTTCGAGGGGAAGGA	2nd Transf. Fig. 2C, 5'	
TTGCGCAATCAATGACG	2nd Transf. Fig. 2C, 3'	
CGTATCTTTGTTTACTTTTC	3' diagnostic (mut.), Fig. 2C	
CGTATCTTTGTTTACTTGTA	3' diagnostic (WT.) , Fig. 2C	
GTAAGAGAAAAAGGTATTGG	5' diagnostic , Fig. 2C	
		
GTTTCCCGATATAGAAAACTCAGTGGCTGTGTTACGTGGC	2nd Transf. Fig. 2D, 5'	
CCGTACAGCTATCGTTTCAGG	2nd Transf. Fig. 2D, 3'	
CCCGATATAGAAAACTCAG	3' diagnostic (mut.), Fig. 2D	
CCC GAT ATA GAA AAC TAA T	3' diagnostic (WT.) , Fig. 2D	
GTACCCGTACAGCTATCGTTTCAGG	5' diagnostic , Fig. 2D	
		
GTACCCGTACAGCTATCGTTTCAGG	amplify UCS2 from genome as template for error-prone PCR, 5'	
TTTCACCAGTAATGGGTGCCAGTAGG	amplify UCS2 from genome as template for error-prone PCR, 3'	
		
CTATACAAGCCGCTGGATAAAGCTTATTCGTTGATGTCAT	amplify 9 mutation fragment from pS660	
CAAAGGGTGCTCTAGATCTCATCGATGTATTCTGATGCAG	amplify 9 mutation fragment from pS660