DNA packaging in isolated bacterial nucleoids : (chromosomes, DNA tertiary structure, nucleoids, DNA folding, DNA supercoiling, nascent RNA, membrane-DNA)
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Abstract
Properties of nucleoids isolated from Escherichia coli are described. Experiments which investigate the organization of DNA folding and supercoiling in the isolated chromosome are reviewed, and evidence is discussed suggesting a role for certain proteins and nascent RNA molecules in stabilizing the condensed DNA. A model of the conformational organization of the packaged DNA is presented.