Electromagnetic stimulation of a composite scaffold for wound healing
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[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Decellularized tissues have been investigated as natural sources for tissue scaffolds as synthetic mimicry of the natural extracellular matrix is quite difficult. In addition, gold nanomaterials have been studied for their ability to provide nanostructural cues for cell growth as well as their ability to interact with free radicals. Meanwhile, electromagnetic fields (EMFs) have been explored as an adjunct therapy for chronic wounds due to clinical evidence of wound healing enhancement. This dissertation investigated the incorporation of all three in the design and evaluation of an electromagnetically-stimulated composite scaffold comprised of decellularized porcine diaphragm and gold nanomaterials. Composite scaffolds were synthesized and characterized through biocompatibility, cell proliferation, and reactive oxygen species assays. The scaffolds were then subjected to an EMF while in cell culture to determine the cellular effects of this treatment. The results indicated the successful design of a biocompatible composite scaffold capable of free radical level modulation and enhanced cell proliferation, especially when subjected to an EMF. It was also shown that the interaction of the EMF and gold nanomaterials transiently enhanced cell spreading. Future studies are needed to elucidate the specific mechanisms for these effects and to further develop the system as an advanced wound therapy.
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Access is limited to the campus of the University of Missouri--Columbia.