Core-based hyperbranched polyethyleneimine coating in capillary electrophoresis
No Thumbnail Available
Authors
Meeting name
Sponsors
Date
Journal Title
Format
Thesis
Subject
Abstract
In capillary electrophoresis (CE), chemical and dynamic modification of a fused silica surface can be used to control the electroosmotic flow (EOF), to minimize the interaction of analytes with the capillary surface and to modify the selectivity. , core-based hyperbranched polyethyleneimine (CHPEI) polymer utilized as either a static or dynamic coating in this study. A CHPEI-coated capillary was simply constructed in a rinsing fashion or by adding a small amount of CHPEI in a running buffer. In CHPEI-coated capillaries, several parameters were studied as follows: (1) EOF as a function of buffer pH; (2) effect of coating media (NaCl solution) concentration; (3) effect of buffer concentration; and (4) stability and reproducibility of the coating. Since CHPEI25-coated capillary provides greater EOF stability, CHPEI25 was chosen for further studies. The performance of CHPEI25 dynamic-, static-, and hybrid-coated capillaries was investigated in the separations of phenols, basic amino acids, B vitamins, aniline and its derivatives. The separation performance of CHPEI25 dynamic-coated capillaries were also compared to that of commercial eCAPTM capillary from Beckman Coulter. It is evident that CHPEI25 dynamic-coated capillary provides significantly improved peak resolution under identical separation conditions compared to that in eCAPTM.
Table of Contents
DOI
PubMed ID
Degree
Ph. D.
Thesis Department
Rights
OpenAccess.
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.