B2 or not B2 : loss of ephrin-B2 in endothelial cells during development, homeostasis, and regeneration
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The microvasculature is a robust and highly adaptive system, responsive to minute physiological and molecular cues. Endothelial cells (ECs) assemble the microvasculature during development through vasculogenesis and angiogenesis. Angiogenesis is the tightly controlled process of forming new vessels. Disruptions in this process are often fatal. Ephrin-B2 is a cell surface molecule that is necessary for angiogenesis during development and is thought to act in capillary sprouting. We show that conditional deletion of ephrin-B2 (ephrin-B2CKO) during development prior to e10.5 leads to disruptions in vascular formation resulting in embryo death within 12-24 hours of tamoxifen injection. Our larger goal was to ask to what extent ECs interact with satellite cells, the resident stem cell of skeletal muscle, during regeneration after acute injury. We know very little about how ECs interact with other cells in their environment during regeneration. Despite efforts to inhibit angiogenesis during regeneration (ephrin-B2CKO and inhibition of VEGFR), we could only disrupt the organization of the regenerated network. Our data suggest that endothelial ephrin-B2 is only necessary during embryogenesis prior to e10.5, and that inhibition of sprouting angiogenesis does not significantly impact muscle regeneration in the adult.
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Ph. D.