Identification, characterization and regulation of Type II estrogen receptors in cultured human pituitary cells
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The presence of estradiol receptors in the cytosol and pellet fractions of homogenized 18-54, SF cells was investigated. Using the protamine sulfate precipitation assay we have demonstrated that these cells, like other cell lines, seem to lack type I estrogen receptors in the cytoplasm but that these are present in the pellet fraction. The cells have what we have called type II-like receptors in the cytoplasm and presumably in the nucleus. The type II receptors in the cytosol and pellet fraction of homogenized 18-54, SF cells seem to differ in binding parameters, specificity and, probably other characteristics. The cytosol receptor is most probably a protein or contains a protein moiety susceptible to proteolytic degradation. It also seems to have an SH-group essential for binding. The regulation of the type II-like receptors in the cytosol is most likely different and independent of the regulation of type I receptors. Both type II receptors may be involved in the regulation of prolactin secretion by steroids and steroid-related compounds.
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