Exploring the subcellular distribution of mRNA in soybean nodule cells : implications for translational regulation and cell identity.
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The intricate anatomy of eukaryotic organisms is orchestrated by cells that share an identical genome yet exhibit significant functional diversity. This diversity arises from the differential transcription of genes and translation of mRNA. Current research has significantly emphasized studying and understanding the complexities of transcriptional and translational regulation. However, some lesser-explored regulatory mechanisms, such as mRNA sub-cellular localization and its impact on translational regulation and protein production, remain understudied. This is especially true in the field of plant science. We addressed this significant gap in knowledge through the study of subcellular localization of transcripts using an emerging high-resolution and high-sensitivity spatial transcriptomic technology, Molecular Cartography, on the soybean nodule, a root organ where plant cells are infected by symbiotic soil nitrogen-fixing bacteria. By doing this, we observed the differential distribution of mRNA between the plant cell's nucleus and the cytosolic compartments. In addition, we analyzed the cytoplasmic distribution of transcripts in the infected cells of the soybean nodule and discussed its importance in regulating gene function and cell state. Our work highlights a less explored post-transcriptional/pre-translational regulatory process that likely impacts cell state, function, and differentiation.
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M.S.