Fermentation process development for efficient biosynthesis of polyhydroxyalkanoate biopolymers from low-cost carbon sources
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This dissertation presents an innovative investigation into the production of polyhydroxyalkanoates (PHA) from waste materials, specifically focusing on waste plastics and cheese whey. The study explores the optimization of fermentation processes for high-yield PHA synthesis, utilizing model compounds such as fatty acids and hydrocarbons, which simulate degraded plastic substrates, and employing Pseudomonas putida and Pseudomonas resinovorans. It demonstrates that medium-chain fatty acids, particularly decanoic acid (C10), are most effective in maximizing both cell growth and PHA content, while longer-chain fatty acids show limited fermentability. The study also investigates the use of CO2 plasma-treated polyethylene (PE) films, particularly Vir-OIL, as oil substrates, revealing high fermentability and significant PHA yield enhancement through co-culture fermentation. The dissertation further delves into PHA synthesis from cheese whey, employing a two- stage fermentation process. The first stage involves anaerobic fermentation for mixed acid production, with the second stage utilizing P. putida for PHA synthesis. It highlights the critical role of lactic and acetic acids as carbon sources, optimizing PHA accumulation. The study also examines the impact of co-substrates like glycerol and acetic acid on PHB production, uncovering their significant influence on cell growth and polymer accumulation. The study emphasizes the potential of waste-derived substrates in sustainable bioplastic production. This work contributes to advancing the circular economy by offering a promising solution to the global plastic waste crisis, positioning waste as a valuable resource to produce high-performance bioplastics.
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Ph. D.