Deformation mapping of fibronectin fibrils using digital image correlation
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Abstract
Bone cells produce large amounts of extracellular matrix (ECM) proteins that form a fibrillar scaffold on which bone mineral is deposited. Prior work has shown that fibronectin fibrils are one of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of fibronectin by osteoblasts is a highly dynamic process, in which the forming fibronectin fibril network is continually stretched, contracted and moved via the activity of motile cells. This study has developed an automated Digital Image Correlation based technique for determining the displacements and strains experienced by the fibrils during the assembly process. Fibril motion is tracked using a time lapse sequence of images and the motion kinematics are determined using an image cross-correlation method. The method is implemented in Matlab and compared to manual calculations. Furthermore, we have studied the motion of a set of 20 fibrils in order to predict fibril kinematics.
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Introduction -- Description of the program -- Results and observations -- Conclusion and future work
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M.S.