Peptide amphiphile micelles vaccines for cell-mediated immune response induction
No Thumbnail Available
Authors
Meeting name
Sponsors
Date
Journal Title
Format
Thesis
Subject
Abstract
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI SYSTEM AT REQUEST OF AUTHOR.] Vaccinology research looks to improve upon current formulations by enhancing their immunogenicity and safety. In the quest for an alternative solution, researchers have turned to subunit vaccines due to their potential scalability, price, and increased safety. Peptide amphiphile micelles (PAMs), have been found to be an exciting delivery vehicle to enhance the immunogenic potential of subunit vaccines. This research focuses on exploring the capability of PAMs to induce cell-mediated immune responses to complement previous research which has demonstrated their antibody-mediated induction capacity. Materials characterization studies confirmed that peptide amphiphiles (PAs) comprised of a lipidated ovalbumin T cell antigen (OVA[CytoT] - SIINFEKL) form spherical and short cylindrical PAMs which can be complexed with CpG adjuvant via electrostatic interactions. Ex vivo analysis of animals vaccinated with CpG-modified antigenic PAMs showed an increase in OVA[CytoT]-specific CD8[+] T cell populations in draining lymph nodes in a charge-dependent manner over traditional peptide vaccine formulations. Additionally, studies investigating lymphocytes re-stimulated with OVA[CytoT] peptide in vitro showed a similar response to the ex vivo experiment with less charged micelles inducing greater IFN-[gamma]+ CD8[+] T cells in both the draining lymph node and spleen. This research demonstrated the ability of adjuvant-associated PAMs to improve antigen-specific cell-mediated immune responses and demonstrates a need for future work determining the importance of creating fundamental design rules for charge complexed multicomponent micelle vaccines
Table of Contents
DOI
PubMed ID
Degree
M.S.
Thesis Department
Rights
Access to files is restricted to the campuses of the University of Missouri