Influence of redox potential on cell growth and metabolism in pH-controlled continuous butanol fermentation
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The objectives of this thesis were to characterize the kinetics of acetone-butanol fermentations using Clostridium acetobutylicum and to investigate the relationship of redox potential on the cell growth and metabolism in order to improve the overall performance of this fermentation. The kinetic relationships between the cell growth, product formation and substrate consumption by C. acetobutylicum were characterized using batch and continuous fermentations. The batch fermentation progressed rapidly and was completed in 40 hours and resulted in the production of 3.7 g acetone/liter and 8.6 g butanol/liter. In continuous fermentation, highest yield and productivity of butanol were -1 obtained at a dilution rate of 0.02 hr. However, high butanol concentrations seemed to have an adverse effect on the cell activity. Redox potential was found to be an important indicator of cellular activity and solvent production. The cells consumed much more lactose and resulted into high yield and productivity of butanol at lower redox potential values. It is a useful indicator of the state of the fermentation process.
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