Characterization of SF-hERR[beta] repression of human estrogen receptor alpha and estrogen receptor-beta transcriptional activities
No Thumbnail Available
Authors
Meeting name
Sponsors
Date
Journal Title
Format
Thesis
Subject
Abstract
Discovery of new ways to modulate estrogen actions through estrogen receptor is always exciting because of the importance of estrogen and ERs in many human diseases. In this dissertation, we report our discovery of the cross-talk between SF-hERRb and hERs and the mechanism of this cross-talk. We found that SF-hERR[beta] is able to repress the transcriptional activities of hER[alpha] and hER[beta] in various cell lines. This inhibitory effect of SF-hERR[beta] is not through alterations of estradiol binding to hERs. SF-hERRb does not inhibit hERs through direct Estrogen Response Element (ERE) competition. SF-hERR[beta] induces no alterations in hERs protein concentrations. SF-hERR[beta] inhibition of hERs is not through competition/sequestration for PGC-1[alpha], though PGC-1[alpha] can be involved. The A/B domain of hERa and the A/B and D domains of SF-hERR[beta] are required for this inhibition. We determined that SF-hERR[beta] forms complexes with hER[alpha]/hER[beta]. In addition, DY131, a hERR[beta]/hERR[gamma] specific agonist can inhibit hER and SF-hERR[beta] positive human breast cancer MCF-7 cell growth. These findings provide us novel approaches to regulate hERs activities which may lead to discovery of new therapeutic targets for ER-dependent diseases.
Table of Contents
DOI
PubMed ID
Degree
Ph. D.
Thesis Department
Rights
OpenAccess.
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.