Browsing by Thesis Department "Cell Biology and Biophysics (UMKC)"
Now showing items 1-20 of 48
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3-Hydroxy-3-methylglutaryl-coenzyme A lyase: investigation of cysteines mediating intersubunit disulfide formation and regulation by thiol/disulfide exchange and discovery of an extramitochondrial homolog
(University of Missouri -- Kansas City, 2012-01-18)3-Hydroxy-3-methylglutaryl-Coenzyme A lyase catalyzes the cleavage of 3-hydroxy- 3-methylglutaryl-Coenzyme A into acetoacetate and acetyl-Coenzyme A, a key reaction in ketogenesis and leucine catabolism. Previous work ... -
APP∙CNTN Complex Architecture is Conserved Throughout the Vertebrate Lineage
(2020)Contactins (CNTNs) are a group of up to six cell adhesion molecules (CAMs) of the immunoglobulin (Ig) superfamily found throughout the metazoan lineage. CNTN architecture is broadly conserved, and these proteins are composed ... -
Biochemical and Structural Studies of the Role of FKBP(L)s in the fly and Mammalian Circadian Cycle
(University of Missouri -- Kansas City, 2017)FK506 binding-like proteins (FKBPLs) are proteins that structurally resemble the immunophilins. Their functions in cell signaling include protein folding, molecular trafficking, RNA splicing and cell cycle regulation ... -
Calculating the structure-based phylogenetic relationship of distantly related homologous proteins utilizing maximum likelihood structural alignment combinatorics and a novel structural molecular clock hypothesis
(2013)Dendrograms establish the evolutionary relationships and homology of species, proteins, or genes. Homology modeling, ligand binding, and pharmaceutical testing all depend upon the homology ascertained by dendrograms. ... -
Determining the differential roles of the dock family of GEFs in drosophila development
(2015-05-19)The evolutionarily conserved Dock proteins function as unconventional guanine nucleotide exchange factors (GEFs). Upon binding to ELMO (Engulfment and cell motility) proteins, Dock-ELMO complexes activate the Rho family ... -
Disconnecting a Role for DNA Repair in the Pathogenicity of the Human pathogen Cryptococcus neoformans
(2014)C. neoformans is an opportunistic fungal pathogen causing fatal meningitis and lung infections. Worldwide every year around 625,000 deaths occur due to Cryptococcosis. A basic understanding of Cryptococcus virulence ... -
Discovery of Enzymes Responsible for an Alternate Mevalonate Pathway in Haloferax volcanii
(2014-09-30)Archaea are a distinct evolutionary domain of microorganisms that contain isoprenoids in ether linkages to glycerol rather than the ester linked fatty acids that characterize membranes of bacteria or eukaryotes. Radiolabeling ... -
Effect of TIMBD on Neurotoxicity Associated with HIV-1 Gp120 and Tat
(University of Missouri -- Kansas City, 2018)Human Immunodeficiency Virus (HIV)-associated neurocognitive disorder (HAND) is one of the undermining disorders that affects majority of HIV infected patients. Patients with HAND suffer from both cognitive and motor ... -
Effects of Light on BDBT Puncta Formation in the Drosophila Eye Reveal Involvement of BDBT with Color Preference Behavior and Distinct Nuclear Localization Pathways for DBT and PER
(2021)In this dissertation, the research focus is on elucidating the mechanism behind BRIDE OF DOUBLETIME (BDBT) puncta/foci formation. During this process it was discovered that both the circadian and visual photoreceptors ... -
The expression and regulation on antimicrobial peptide genes in insects: studies in the tobacco hornworm manduca sexta
(University of Missouri--Kansas City, 2011-05-10)Insects have evolved an efficient innate immune system to combat microbes. Most of our knowledge of insect immunity comes from studies with dipteran and lepidopteran insects, such as the fruit fly Drosophila melanogaster, ... -
The function of Drosophila Integrin-Dim7-Elmo-Mbc→Rac signaling pathway in the muscle attachment formation and maintenance
(2013)The Engulfment and Cell Motility (Elmo)-(Myoblast city) Mbc→Rac signaling pathway is evolutionarily conserved from C. elegans to vertebrates and is essential for many developmental processes, including phagocytosis and ... -
A genetic linkage map of the fungus phycomyces blakesleeanus for gene identification by map-based cloning
(University of Missouri--Kansas City, 2012)The Mucoromycotina is one of the basal lineages in the fungi, all of which are poorly understood. Mucormycosis is a life threatening infection caused by fungi of the order Mucorales, the most common species of which is ... -
A Genetic Screen for Tribbles Suppressors Identifies the E3 Ubiquitin Ligase Neuralized as a Novel Target
(University of Missouri -- Kansas City, 2017)In this dissertation, I used the Drosophila wing as a model tissue for a genetic screen to identify novel targets of Tribbles. Drosophila Tribbles is the founding member of a family of pseudokinases conserved between ... -
The herpes simplex virus virion host shutoff protein is targeted to mRNA cleavage sites through interactions with components of the host translational apparatus
(University of Missouri--Kansas City, 2011-01-20)The virion host shutoff protein (Vhs) is a herpes simplex virus (HSV) protein involved in early shutoff of the host cell. It is a component of the infecting virion, located in the tegument region, that works by rapidly ... -
HIV-1 gp120 and Methamphetamine-mediated Induction of Proinflammatory Cytokines/chemokines and Oxidative Stress in Astrocytes: Implications in Neuroinflammation
(2014-04-02)HIV associated neurocognitive disorder (HAND) remains a major concern for patients infected with HIV. The viral envelope protein, gp120 has been extensively studied and some of its neurotoxic effects are due to the increased ... -
Hyper-Plastic Structural Evolution Of The PEX Domain - A Model Of Evolutionary Exaptation And Neofunctionalization At The Molecular Level
(University of Missouri--Kansas City, 2017)With the advent of sophisticated genetic, biophysical and in silico technology an enormous amount of information is being generated regarding the structural, biochemical and physiological aspects of proteins. Tertiary ... -
Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1
(University of Missouri–Kansas City, 2016)The motor proteins dynein and kinesin are fascinating biological machines which, like vehicles in a city, move various cellular cargoes along cytoskeletal microtubules (MT). In filamentous fungi, these motors are important ... -
Identification of motifs in the tribbles kinase required for substrate interactions
(2013)The gene tribbles encodes an atypical kinase that is evolutionarily conserved with important roles in regulation of cell signaling. In Drosophila melanogaster Tribbles (Trbl) directs the proteasomal degradation of the ... -
Identification of Novel Regulatory Genes in Acetaminophen Induced Hepatocyte Toxicity by a Genome-Wide CRISPR/Cas9 Screen
(University of Missouri -- Kansas City, 2018)Acetaminophen (APAP) is a commonly used analgesic responsible for over 56,000 overdose-related emergency room visits annually. A long asymptomatic period and limited treatment options result in a high rate of liver ... -
Independent Functions Of Clueless In Differential Integrin Secretion And Mitochondrial Quality Control In Drosophila Muscle
(2015)Drosophila Clueless (Clu) and its conserved orthologs are known for their role in the prevention of mitochondrial clustering. Here, we uncover its independent roles in the delivery of integrin subunits and the regulation ...