Search
Now showing items 1-5 of 5
Optical stimulation of quantal exocytosis on transparent microchips
(University of Missouri--Columbia, 2007)
Photorelease of caged Ca²⁺ is a uniquely powerful tool to study the dynamics of Ca²⁺-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent ...
Nano- and micro-scale studies of exocytosis
(University of Missouri--Columbia, 2007)
Neurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane ...
Study of low noise high throughput microchip device for electrochemical measurement from single cells
(University of Missouri--Columbia, 2012)
We are developing transparent multi- electrochemical electrode arrays on microchips in order to automate measurement of quantal exocytosis of oxidizable transmitter from individual vesicles. In order to achieve low noise ...
Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips
(University of Missouri--Columbia, 2013)
An electrochemical microelectrode located immediately adjacent to a single neuroendocrine cell can record spikes of amperometric current that result from quantal exocytosis of oxidizable transmitter from individual vesicles. ...
Automated detection of amperometric spikes resulting from quantal exocytosis and estimation of spike and pre-spike foot signal parameters
(University of Missouri--Columbia, 2018)
Electrochemical microelectrodes can detect single-vesicle release events as "spikes" of amperometric current. We developed a template based "matched-filter" approach that performs least squares fit of a library of templates ...