Search
Now showing items 1-20 of 26
Ribozymes and aptamers in the RNA world, and in synthetic biology
(University of Missouri--Columbia, 2016)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The RNA world hypothesis postulates that Ribonucleic Acids (RNA) may have provided functions of catalysis and genetic information storage during the ...
Discovery and characterization of regulatory mechanisms affecting the heteromeric acetyl-coenzyme a carboxylase in Arabidopsis
(University of Missouri--Columbia, 2016)
Fatty acid biosynthesis (FAS) is an essential metabolic pathway used by all organisms to generate fatty acids. A staple component of this pathway is the enzyme acetyl-CoA carboxylase (ACCase), which catalyzes the committed ...
Advances in aptamer evolution and engineering
(University of Missouri--Columbia, 2016)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Aptamers are single-stranded nucleic acids that fold into unique three-dimensional shapes that allow them to bind with high affinity and specificity ...
Structural studies of glyceraldehyde-3-phosphate dehydrogenase complexes and the E. coli PutA DNA binding domain
(University of Missouri--Columbia, 2006)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that catalyzes the formation of 1,3-bisphosphoglycerate from glyceraldehyde-3-phosphate. We have solved a high-resolution (1.75 Å) structure of a ...
S-RNase proteins: functional studies of the 120kDa glycoprotein and SRNase oligomerization
(University of Missouri--Columbia, 2005)
Flowering plants control fertilization through pollen-pistil interactions. Self-incompatibility (SI) is a well-studied pollen-pistil interaction that promotes cross-pollination. SI is controlled by a multi-haplotype locus ...
Examination of metabolic and regulatory networks of desulfovibrio species
(University of Missouri--Columbia, 2004)
The sulfate-reducing bacteria are a morphologically diverse group of organisms characterized by the ability to couple the enzymatic reduction of sulfate to energy production and growth. This metabolic activity has profound ...
Characterization and functional significance of Msc2p & Zrg17p, which form a zinc transport complex in the endoplasmic reticulum of Saccharomyces cerevisiae
(University of Missouri--Columbia, 2004)
We demonstrate a novel interaction between two members of the cation diffusion facilitator (CDF) family in Saccharomyces cerevisiae: Msc2p and a newly recognized CDF family member, Zrg17p. Both Msc2p and Zrg17p have been ...
Characterization and functional analysis of the P2Y₂R gene promoter
(University of Missouri--Columbia, 2006)
Extracellular nucleotides can bind to the P2Y₂R and modulate proliferation and migration of smooth muscle cells, which is known to be involved in intimal hyperplasia that accompanies atherosclerosis and post-angioplasty ...
Structural and functional studies of proline catabolic enzymes
(University of Missouri--Columbia, 2007)
Catabolism is the oxidation of organic nutrients into simple end molecules to extract energy. For proline, the catabolic pathway involves two enzymes, Lproline dehydrogenase (PRODH, EC 1.5.99.8) and L-[delta]¹-pyrroline- ...
Structure of an antigen-binding fragment bound to stem-loop DNA and crystallization of recombinant haemophilus influenzae e(P4) acid phosphatase
(University of Missouri--Columbia, 2006)
DNA-1 and 11F8 are anti-ss DNA antibodies derived from autoimmune lupus-prone mice. They are very similar to each other in terms of CDR sequence and preference for binding T-rich ss DNA. G1-17 is an oligonucleotide identified ...
Kinase-interacting FHA domain of kinase associated protein phosphatase : phosphopeptide interactions and NMR-detected dynamics
(University of Missouri--Columbia, 2007)
FHA domains are phosphoThr recognition modules found in diverse signaling proteins. Kinase-associated protein phosphatase (KAPP) from Arabidopsis employs its FHA domain in its negative regulation of some receptor-like ...
A custom oligonucleotide microarray analysis as a tool for dissecting soybean-bradyrhizobium japonicum nodule senescence
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean root nodules are symbiotic organs housing Bradyrhizobium japonicum, a soil bacterium that reduces atmospheric nitrogen to ammonium. The bacterium ...
Phenotypic modulation of vascular smooth muscle cells : evidence for a role of the G protein-coupled P2y2 receptor
(University of Missouri--Columbia, 2008)
G protein-coupled receptors regulate two major functions of vascular smooth muscle cells (SMCs) - the vasomotor contractile response and the proliferative response. The contractile phenotype of SMCs has mainly been studied ...
Physiologic and metabolic interactions in the soybean/bradyrhizobium japonicum symbiosis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The agronomically vital, nitrogen-fixing symbioses between soybean and the soil bacterium Bradyrhizobium japonicum is a model system for the study of ...
A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
(University of Missouri--Columbia, 2008)
For more than 3 decades, the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several ...
Substrate activation in the urate oxidase reaction
(University of Missouri--Columbia, 2008)
The enzyme urate oxidase (UO) is involved in nitrogen metabolism of many organisms. Urate oxidation is carried out by UO without the aid of a metal ion or cofactor. A catalytic diad has previously been proposed to be ...
Protein phosphorylation regulation in Arabidopsis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Protein phosphorylation and dephosphorylation are fundamental mechanisms involved in many cell-signaling events. Coordinated actions of protein kinases ...
Molecular and cell biological studies of mammalian zinc transporters
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Zinc is an essential trace element in all organisms. The importance of the hZIP4 human zinc transporter is highlighted by its causative role in a ...
Regulation of Nrf2 by a keap1-dependent E3 ubiquitin ligase
(University of Missouri--Columbia, 2007)
Oxidative damage to biological macromolecules has been implicated in diverse pathophysiological processes, including cancer, neurodegeneration, aging and cardiovascular disease. The Nrf2 transcription factor regulates ...
Understanding Arabidopsis ion homeostasis in the post-genomic era : assigning function to two proteins involved in iron metabolism
(University of Missouri--Columbia, 2006)
Two projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 ...