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Protein phosphorylation regulation in Arabidopsis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Protein phosphorylation and dephosphorylation are fundamental mechanisms involved in many cell-signaling events. Coordinated actions of protein kinases ...
Physiologic and metabolic interactions in the soybean/bradyrhizobium japonicum symbiosis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The agronomically vital, nitrogen-fixing symbioses between soybean and the soil bacterium Bradyrhizobium japonicum is a model system for the study of ...
Structural studies of glyceraldehyde-3-phosphate dehydrogenase complexes and the E. coli PutA DNA binding domain
(University of Missouri--Columbia, 2006)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that catalyzes the formation of 1,3-bisphosphoglycerate from glyceraldehyde-3-phosphate. We have solved a high-resolution (1.75 Å) structure of a ...
Molecular and cell biological studies of mammalian zinc transporters
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Zinc is an essential trace element in all organisms. The importance of the hZIP4 human zinc transporter is highlighted by its causative role in a ...
Understanding Arabidopsis ion homeostasis in the post-genomic era : assigning function to two proteins involved in iron metabolism
(University of Missouri--Columbia, 2006)
Two projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 ...
Structure of an antigen-binding fragment bound to stem-loop DNA and crystallization of recombinant haemophilus influenzae e(P4) acid phosphatase
(University of Missouri--Columbia, 2006)
DNA-1 and 11F8 are anti-ss DNA antibodies derived from autoimmune lupus-prone mice. They are very similar to each other in terms of CDR sequence and preference for binding T-rich ss DNA. G1-17 is an oligonucleotide identified ...
The role of P2Y₂ nucleotide receptors in vascular inflammation
(University of Missouri--Columbia, 2007)
Extracellular nucleotides act on P2 receptors to regulate vascular tone. Large amounts of extracellular nucleotides are released at the sites of tissue injury and may play an important role in the vascular inflammation. ...
Structural and functional studies of proline catabolic enzymes
(University of Missouri--Columbia, 2007)
Catabolism is the oxidation of organic nutrients into simple end molecules to extract energy. For proline, the catabolic pathway involves two enzymes, Lproline dehydrogenase (PRODH, EC 1.5.99.8) and L-[delta]¹-pyrroline- ...
Regulation of Nrf2 by a keap1-dependent E3 ubiquitin ligase
(University of Missouri--Columbia, 2007)
Oxidative damage to biological macromolecules has been implicated in diverse pathophysiological processes, including cancer, neurodegeneration, aging and cardiovascular disease. The Nrf2 transcription factor regulates ...
Kinase-interacting FHA domain of kinase associated protein phosphatase : phosphopeptide interactions and NMR-detected dynamics
(University of Missouri--Columbia, 2007)
FHA domains are phosphoThr recognition modules found in diverse signaling proteins. Kinase-associated protein phosphatase (KAPP) from Arabidopsis employs its FHA domain in its negative regulation of some receptor-like ...
Characterization and functional analysis of the P2Y₂R gene promoter
(University of Missouri--Columbia, 2006)
Extracellular nucleotides can bind to the P2Y₂R and modulate proliferation and migration of smooth muscle cells, which is known to be involved in intimal hyperplasia that accompanies atherosclerosis and post-angioplasty ...
Substrate activation in the urate oxidase reaction
(University of Missouri--Columbia, 2008)
The enzyme urate oxidase (UO) is involved in nitrogen metabolism of many organisms. Urate oxidation is carried out by UO without the aid of a metal ion or cofactor. A catalytic diad has previously been proposed to be ...
A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
(University of Missouri--Columbia, 2008)
For more than 3 decades, the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several ...
Phenotypic modulation of vascular smooth muscle cells : evidence for a role of the G protein-coupled P2y2 receptor
(University of Missouri--Columbia, 2008)
G protein-coupled receptors regulate two major functions of vascular smooth muscle cells (SMCs) - the vasomotor contractile response and the proliferative response. The contractile phenotype of SMCs has mainly been studied ...
A custom oligonucleotide microarray analysis as a tool for dissecting soybean-bradyrhizobium japonicum nodule senescence
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean root nodules are symbiotic organs housing Bradyrhizobium japonicum, a soil bacterium that reduces atmospheric nitrogen to ammonium. The bacterium ...
Signals affecting the urease status of plant-associated bacteria, Methylobacterium spp
(University of Missouri--Columbia, 2007)
Pink-Pigmented Facultative Methylotrophic bacteria (PPFMs, Methylobacterium spp.) have been found to be the most abundant microorganisms among phylloplane microflora, and have been recovered from all plants examined. I ...