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Establishing a foundation for large DNA transfer to artificial minichromosomes and B insert platforms in maize
(University of Missouri--Columbia, 2019)
In plants, conventional genetic engineering methods limit the number of available traits that could potentially improve the quality of agriculture. Agrobacterium-mediated transformation and biolistic bombardment are tools ...
Development of an amenable system for site-specific addition to a maize chromosome
(University of Missouri--Columbia, 2019)
Currently, transgenic maize is produced by random integration of a transgenes into the plant. This works for single genes, but not as well for multiple traits. Identifying plants that contain several transgenes becomes a ...
Mechanisms of polar growth in the alphaproteobacterial order rhizobiales
(University of Missouri--Columbia, 2019)
All bacteria elongate and divide to faithfully reproduce their cell shape. Understanding the mechanisms that drive bacterial morphology requires an intimate knowledge of how the cell wall is synthesized. During cell ...
From unique killers to a jumbo genome - isolation and characterization of phages that infect the plant pathogen, Agrobacterium tumefaciens
(University of Missouri--Columbia, 2019)
against A. tumefaciens C58. These phages come in different shapes and sizes--from T7-like phages with podoviral morphology and isometric heads to T4-like phages with myoviral morphology and a contractile tail--and exhibit varying host ranges and killing...
Effects of prescribed fire and timber harvest on terrestrial salamander abundance, behavior, and microhabitat use
(University of Missouri--Columbia, 2014)
from 0.4 to 1.6 salamanders per square meter. I found that salamanders were most likely to be on the forest floor surface during or following rainfall, and that they were more likely to be in leaf litter than under cover objects if it had recently...
Approaches for unveiling the kinetic mechanisms of voltage gated ion channels in neurons
(University of Missouri--Columbia, 2019)
Ionic currents drive cellular function within all living cells to perform highly specific tasks. For excitable cells, such as muscle and neurons, voltage-gated ion channels have finely tuned kinetics that allow the ...
The secret lives of African forest elephants: using genetics, networks, and telemetry to understand sociality
(University of Missouri--Columbia, 2012)
Knowledge of social structure is important to understanding the overall evolution and behavioral ecology of a species as it ultimately influences spatial patterns and gene flow. This dissertation focuses on the social ...
The Agrobacterium tumefaciens min system contributes to the regulation of cell division
(University of Missouri--Columbia, 2016)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] In A. tumefaciens, the essential FtsZ protein is located at the growth pole before shifting to the mid-cell right before division. Recruitment of FtsZ ...
PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
(University of Missouri--Columbia, 2018)
Understanding how bacterial cells expand their cell walls is an important question with relevance to development of antibiotics. While many studies have focused on the regulation of bacterial elongation utilizing lateral ...
Tales of an 'invisible' life stage : survival and physiology among terrestrial juvenile ambystomatid salamanders
(University of Missouri--Columbia, 2019)
The vital rates (e.g., survival, growth, and reproduction) of distinct life stages within a species are known to influence the growth and persistence of populations. As such, studies describing stage-specific vital rates, ...
The impacts of partner abundance on benefits from facultative pollination mutualism
(University of Missouri--Columbia, 2010)
Partner abundance affects costs and benefits in obligate mutualisms, but its role in facultative partnerships is less clear. I investigated this topic in a small alpine pollination web in the Colorado Rocky Mountains ...
Seasonality and elevational migration in an Andean bird community
(University of Missouri--Columbia, 2010)
Life history strategies of many birds in the Neotropics remain poorly known. In particular, details on the seasonal movements of individuals and populations within the Neotropics remain largely unknown, including short-distance ...