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Establishing a foundation for large DNA transfer to artificial minichromosomes and B insert platforms in maize
(University of Missouri--Columbia, 2019)
In plants, conventional genetic engineering methods limit the number of available traits that could potentially improve the quality of agriculture. Agrobacterium-mediated transformation and biolistic bombardment are tools ...
Drug resistance in D. discoideum: isolation of 4-nitroquinoline 1-oxide resistant mutants
(University of Missouri--Columbia, 2005)
The drug 4-nitroquinoline 1-oxide (4NQO) displays both carcinogenic and antitumor effects, a well known characteristic of many chemotherapeutic drugs. In addition 4NQO shares a similar operating mechanism with the commonly used chemotherapeutic drug...
Behavioral choice and demographic consequences of wood frog habitat selection in response to land use
(University of Missouri--Columbia, 2007)
throughout the circular experimental timber harvest arrays (164 m radius) by using standard radiotelemetry techniques. Prior to timber harvest, I found that wood frogs were not distributed equally throughout oak-hickory forest. Adults used drainages as non...
Development of an amenable system for site-specific addition to a maize chromosome
(University of Missouri--Columbia, 2019)
Currently, transgenic maize is produced by random integration of a transgenes into the plant. This works for single genes, but not as well for multiple traits. Identifying plants that contain several transgenes becomes a ...
Identification and characterization of M cells in the mammalian conjunctiva
(University of Missouri--Columbia, 2007)
The M cell is a morphologically and functionally distinct epithelial cell found in the follicle associated epithelium (FAE) above organized lymphoid tissue at distinct locations along mucosal surfaces. By sampling antigens ...
Mapping nif genes of Rhodobacter capsulatus, and characterization of a mutant with a pleiotropic defect in nitrogen metabolism
(University of Missouri--Columbia, 1988)
Mutations affecting the ability of the photosynthetic bacterium Rhodobacter capsulatus to fix nitrogen (Nif- ) have previously been mapped into six linkage groups (I-VI) by transduction with gene transfer agent (GTA). ...
Uncovering the genetic basis of seed amino acid composition in arabidopsis using a multi-omics integrative approach
(University of Missouri--Columbia, 2021)
Seeds are a vital source of protein in the diet of humans and livestock. However, protein composition in the seed is low, comprising about 10 percent of the total composition in the seed. Additionally, protein quality in ...
Mechanisms of polar growth in the alphaproteobacterial order rhizobiales
(University of Missouri--Columbia, 2019)
All bacteria elongate and divide to faithfully reproduce their cell shape. Understanding the mechanisms that drive bacterial morphology requires an intimate knowledge of how the cell wall is synthesized. During cell ...
Regulation of ceramide synthase 1 in cellular stress response
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Ceramides are intimately involved in a wide range of cellular processes. In mammals, de novo ceramide synthesis is catalyzed by the 6 ceramide synthase ...
Gray treefrog breeding site selection and offspring performance in response to forest management
(University of Missouri--Columbia, 2007)
Amphibian diversity and abundance are generally reduced following clearcut logging. These impacts often last for decades, but little is known about why diversity and abundance are depressed. I examined gray treefrog breeding ...
Behavioral endocrinology of female gray treefrogs, Hyla versicolor, in response to acoustic stimulation
(University of Missouri--Columbia, 2008)
Particularly in organisms with a limited breeding period, females must rely on both external and physiological cues to regulate the phenology of reproduction and behavior. I investigated the relationship between male ...
From unique killers to a jumbo genome - isolation and characterization of phages that infect the plant pathogen, Agrobacterium tumefaciens
(University of Missouri--Columbia, 2019)
against A. tumefaciens C58. These phages come in different shapes and sizes--from T7-like phages with podoviral morphology and isometric heads to T4-like phages with myoviral morphology and a contractile tail--and exhibit varying host ranges and killing...
Coupling, membrane conductance, and ion channel mRNA profiles in the establishment and maintenance of network activity in the crustacean cardiac ganglion
(University of Missouri--Columbia, 2021)
Neural networks produce critical rhythmic behaviors throughout an animal's lifespan, despite growth, differing environments, and changes in physiological state. This requires networks which balance stability in their ...
Role of non-hypophototropic hypocotyl[3] (NPH[3]) in regulation of phototropism in Arabidopsis
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Light is arguably one of the most important environmental factor that not only provides the plant with its energy needs but also cues to modulate its ...
A study of the role of the Atg8 protein in autophagic vesicle formation
(University of Missouri--Columbia, 2009)
) The deconjugation of Atg8 from PE is essential for completion of autophagosome formation (2) Deconjugation of Atg8-PE requires Atg4 protease; (3) presence of Atg18 protein is essential for deconjugation during starvation. Here we present substantial evidence...
Effects of prescribed fire and timber harvest on terrestrial salamander abundance, behavior, and microhabitat use
(University of Missouri--Columbia, 2014)
from 0.4 to 1.6 salamanders per square meter. I found that salamanders were most likely to be on the forest floor surface during or following rainfall, and that they were more likely to be in leaf litter than under cover objects if it had recently...
A multi-scale investigation of ecologically relevant effects of agricultural runoff on amphibians
(University of Missouri--Columbia, 2008)
Modification of landscapes for agricultural production can introduce agrochemicals into surface waters and degrade aquatic habitats used by many amphibians for breeding and larval development. Although many ecotoxicological ...
Approaches for unveiling the kinetic mechanisms of voltage gated ion channels in neurons
(University of Missouri--Columbia, 2019)
Ionic currents drive cellular function within all living cells to perform highly specific tasks. For excitable cells, such as muscle and neurons, voltage-gated ion channels have finely tuned kinetics that allow the ...
The secret lives of African forest elephants: using genetics, networks, and telemetry to understand sociality
(University of Missouri--Columbia, 2012)
Knowledge of social structure is important to understanding the overall evolution and behavioral ecology of a species as it ultimately influences spatial patterns and gene flow. This dissertation focuses on the social ...
The Agrobacterium tumefaciens min system contributes to the regulation of cell division
(University of Missouri--Columbia, 2016)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] In A. tumefaciens, the essential FtsZ protein is located at the growth pole before shifting to the mid-cell right before division. Recruitment of FtsZ ...