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Establishing a foundation for large DNA transfer to artificial minichromosomes and B insert platforms in maize
(University of Missouri--Columbia, 2019)
In plants, conventional genetic engineering methods limit the number of available traits that could potentially improve the quality of agriculture. Agrobacterium-mediated transformation and biolistic bombardment are tools ...
Drug resistance in D. discoideum: isolation of 4-nitroquinoline 1-oxide resistant mutants
(University of Missouri--Columbia, 2005)
The drug 4-nitroquinoline 1-oxide (4NQO) displays both carcinogenic and antitumor effects, a well known characteristic of many chemotherapeutic drugs. In addition 4NQO shares a similar operating mechanism with the commonly used chemotherapeutic drug...
Behavioral choice and demographic consequences of wood frog habitat selection in response to land use
(University of Missouri--Columbia, 2007)
throughout the circular experimental timber harvest arrays (164 m radius) by using standard radiotelemetry techniques. Prior to timber harvest, I found that wood frogs were not distributed equally throughout oak-hickory forest. Adults used drainages as non...
Identification and characterization of M cells in the mammalian conjunctiva
(University of Missouri--Columbia, 2007)
The M cell is a morphologically and functionally distinct epithelial cell found in the follicle associated epithelium (FAE) above organized lymphoid tissue at distinct locations along mucosal surfaces. By sampling antigens ...
Development of an amenable system for site-specific addition to a maize chromosome
(University of Missouri--Columbia, 2019)
Currently, transgenic maize is produced by random integration of a transgenes into the plant. This works for single genes, but not as well for multiple traits. Identifying plants that contain several transgenes becomes a ...
Regulation of ceramide synthase 1 in cellular stress response
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Ceramides are intimately involved in a wide range of cellular processes. In mammals, de novo ceramide synthesis is catalyzed by the 6 ceramide synthase ...
The role of terrrestrial habitat in the population dynamics and conservation of pond-breeding amphibians
(University of Missouri--Columbia, 2007)
I used both demographic modeling and experimental field research to evaluate the role of terrestrial habitat in the population dynamics and conservation of pond-breeding amphibians. I began by using literature data to ...
Mechanisms of polar growth in the alphaproteobacterial order rhizobiales
(University of Missouri--Columbia, 2019)
All bacteria elongate and divide to faithfully reproduce their cell shape. Understanding the mechanisms that drive bacterial morphology requires an intimate knowledge of how the cell wall is synthesized. During cell ...
From unique killers to a jumbo genome - isolation and characterization of phages that infect the plant pathogen, Agrobacterium tumefaciens
(University of Missouri--Columbia, 2019)
against A. tumefaciens C58. These phages come in different shapes and sizes--from T7-like phages with podoviral morphology and isometric heads to T4-like phages with myoviral morphology and a contractile tail--and exhibit varying host ranges and killing...
In vitro and in vivo approaches to tumor targeting by phage display
(University of Missouri--Columbia, 2010)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Approximately 30% of breast cancers are linked with the over-expression of ErbB2. Because of its critical role in signal transduction, the progression ...
Characterization of pigment-protein complexes in the cyanobacterium Anacystis Nidulans R2
(University of Missouri--Columbia, 1987)
contain extrinsic phycobilisomes, whereas chloroplasts have integral membrane light-harvesting Chl-protein complexes. We show that cyanobacteria are capable of synthesizing an intrinsic light-harvesting structure (termed CPVI-4) similar in many respects...
Uncovering the genetic basis of seed amino acid composition in arabidopsis using a multi-omics integrative approach
(University of Missouri--Columbia, 2021)
Seeds are a vital source of protein in the diet of humans and livestock. However, protein composition in the seed is low, comprising about 10 percent of the total composition in the seed. Additionally, protein quality in ...
Behavioral endocrinology of female gray treefrogs, Hyla versicolor, in response to acoustic stimulation
(University of Missouri--Columbia, 2008)
Particularly in organisms with a limited breeding period, females must rely on both external and physiological cues to regulate the phenology of reproduction and behavior. I investigated the relationship between male ...
Coupling, membrane conductance, and ion channel mRNA profiles in the establishment and maintenance of network activity in the crustacean cardiac ganglion
(University of Missouri--Columbia, 2021)
Neural networks produce critical rhythmic behaviors throughout an animal's lifespan, despite growth, differing environments, and changes in physiological state. This requires networks which balance stability in their ...
A study of the role of the Atg8 protein in autophagic vesicle formation
(University of Missouri--Columbia, 2009)
) The deconjugation of Atg8 from PE is essential for completion of autophagosome formation (2) Deconjugation of Atg8-PE requires Atg4 protease; (3) presence of Atg18 protein is essential for deconjugation during starvation. Here we present substantial evidence...
Role of non-hypophototropic hypocotyl[3] (NPH[3]) in regulation of phototropism in Arabidopsis
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Light is arguably one of the most important environmental factor that not only provides the plant with its energy needs but also cues to modulate its ...
Approaches for unveiling the kinetic mechanisms of voltage gated ion channels in neurons
(University of Missouri--Columbia, 2019)
Ionic currents drive cellular function within all living cells to perform highly specific tasks. For excitable cells, such as muscle and neurons, voltage-gated ion channels have finely tuned kinetics that allow the ...
PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
(University of Missouri--Columbia, 2018)
Understanding how bacterial cells expand their cell walls is an important question with relevance to development of antibiotics. While many studies have focused on the regulation of bacterial elongation utilizing lateral ...
Ecological genomics of divergence, admixture, and fitness in the smallmouth bass (Micropterus dolomieu)
(University of Missouri--Columbia, 2021)
The Smallmouth Bass (Micropterus dolomieu) is one of the most highly targeted sport fishes in the world. Anglers vie for the opportunity to catch Smallmouth Bass recreationally and competitively, spending billions of dollars ...
The impacts of partner abundance on benefits from facultative pollination mutualism
(University of Missouri--Columbia, 2010)
Partner abundance affects costs and benefits in obligate mutualisms, but its role in facultative partnerships is less clear. I investigated this topic in a small alpine pollination web in the Colorado Rocky Mountains ...