Search
Now showing items 1-20 of 20
Negative biomarkers of sperm quality and male fertility
(University of Missouri--Columbia, 2011)
is growing in popularity in both andrology laboratories and agricultural studs. Here we investigate the application of two biomarkers, the sperm chromatin structure assay (SCSA) and the post-acrosomal ww-domain binding protein (PAWP) to human assisted...
Metabolic programming of a Warburg effect-like phenotype in donor fibroblasts prior to somatic cell nuclear transfer
(University of Missouri--Columbia, 2017)
Gene edited pigs serve as excellent models for biomedicine and agriculture. Currently, the most efficient way to make a reliably-edited transgenic animal is through somatic cell nuclear transfer (SCNT) also known as cloning. This process involves...
Genetic linkage of multiple modifications in the pig
(University of Missouri--Columbia, 2015)
and will continue to have profound effects in the fields of agriculture and human medicine. The current protocols used are effective, but additional enhancements in the efficiencies are desirable. In particular, any added efficiency to modify the genomes...
Improvement of feed efficiency in beef cattle through selection upon residual feed intake (RFI)
(University of Missouri--Columbia, 2010)
Selecting beef cattle which metabolically are more efficient at converting feed into gain or body weight maintenance is one way to reduce feed cost in beef production. Residual feed intake (RFI) is one trait used to identify ...
Determination of fat percentage using three different methods within marbling scores on beef longissimus muscle
(University of Missouri--Columbia, 2010)
Consumers are concerned with fat consumption from meat products and the ability to determine fat has changed with recent technological advances. The objective of this study was to determine fat percentage within marbling ...
Differential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryos
(University of Missouri--Columbia, 2010)
Nuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued ...
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
(University of Missouri--Columbia, 2010)
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos ...
Influence of subclinical hypocalcemia on plasma biochemical parameters, liver histologic changes, and common postpartum diseases in dairy cows
(University of Missouri--Columbia, 2011)
We hypothesized that cows with subclinical hypocalcemia at calving would have greater elevations in liver associated biochemical parameters and non-esterified fatty acid (NEFA) concentrations compared to normocalcemic cows. ...
Effects of Artemisia afra and Artemisia absinthium on fertility of male mice exposed to increased ambient temperatures for 24 hours
(University of Missouri--Columbia, 2010)
Males exposed to elevated temperatures display a decrease in reproductive performance resulting in decreased profit for producers. The use of herbs to alleviate the effects of heat in tropical countries has been documented ...
Abundance of TRIM28, SETDB1, and TP53 mRNA is dynamically regulated during porcine early embryogenesis and is abnormal in preimplantation embryos produced by in vitro fertilization in comparison to in vivo derived and nuclear transfer derived embryos
(University of Missouri--Columbia, 2013)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] In vitro embryo production is important for research in animal reproduction, embryo transfer, transgenics, and cloning. However, in vitro-derived embryos ...
Approaches for alleviating toxic effects of aflatoxin in lactating dairy cows and weanling pigs
(University of Missouri--Columbia, 2010)
Aflatoxin (AF) is a mycotoxin found in feed ingredients used in livestock diets including dairy cattle and pigs. Aflatoxin causes reduced performance, liver damage, increased prevalence of infectious disease, and secretion ...
Structural variation at the KIT locus is responsible for the piebald phenotype in Hereford and Simmental cattle
(University of Missouri--Columbia, 2014)
The Spotted locus is responsible for white spotting (piebald) coat color phenotypes in cattle. Since the Hereford breed is fixed for this phenotype, we postulate that a selective sweep occurred during breed formation to ...
Ruminal degradation of protein and carbohydrate in the domestic and wild ruminant
(University of Missouri--Columbia, 2010)
To optimize growth and health ruminant animals in a confined setting, diets need to be formulated to provide balanced levels of rumen degradable energy and nitrogen (RDN) to maximize microbial growth while also providing ...
Effects of heat stress and fescue toxicosis on the immune system and other physiological parameters
(University of Missouri--Columbia, 2010)
Tall fescue is widely used as pasture grass in the United States, and is the most important cool season grass for grazing animals. Certain fungi also grow on these grasses which produce toxins, resulting in reduced feed ...
Potential diagnostic methods and nutritional changes to combat bovine respiratory disease in receiving cattle
(University of Missouri--Columbia, 2017)
Bovine respiratory disease (BRD) accounts for up to 70 percent of health disruptions and $500 million in associated medical costs and production losses, thus receiving considerable attention from cattle health personnel ...
Transgenerational effects of in utero heat stress on reproduction in pigs
(University of Missouri--Columbia, 2017)
Seasonal infertility caused by high ambient temperatures and humidity during the summer months can have negative effects on the reproduction of female swine. These conditions may cause heat stress leading to large economic ...
Regulation of early trophoblast differentiation and development
(University of Missouri--Columbia, 2010)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Placenta is a transient organ which functions in multiple ways to support the growth and survival of the developing fetus. Placenta plays a critical ...
Determination of allelic expression of H19 in pre- and peri-implantation mouse embryos
(University of Missouri--Columbia, 2013)
H19 is a maternally-expressed imprinted non-coding RNA with tumor suppressor activity. H19 is located in an imprinted cluster which contains the paternally-expressed fetal growth factor Igf2. Monoallelic expression of H19 ...
Alleviation of heat stress with Tasco in dairy cows
(University of Missouri--Columbia, 2010)
The present study determined the impact of Tasco, Ascophyllum nodosum, on heat stress in dairy cows. Thirty-two Holstein cows were utilized in a randomized complete block design, during 63 days in summer 2008. Feed intake, ...
Characterization of progesterone receptor (PGR) mRNA and protein isoforms in the endometrium of cyclic and pregnant pigs
(University of Missouri--Columbia, 2010)
Disappearance of the progesterone receptor (PGR) from the uterine luminal epithelium (LE) is essential for cyclicity and pregnancy in pigs. In humans, three PGR mRNA isoforms (PGR-A, PGR-B and PGR-C) arise from alternative ...