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Metabolic programming of a Warburg effect-like phenotype in donor fibroblasts prior to somatic cell nuclear transfer
(University of Missouri--Columbia, 2017)
Gene edited pigs serve as excellent models for biomedicine and agriculture. Currently, the most efficient way to make a reliably-edited transgenic animal is through somatic cell nuclear transfer (SCNT) also known as cloning. This process involves...
Improved cloning efficiency by chemically induced metabolic reprogramming of donor cells used for porcine somatic cell nuclear transfer
(University of Missouri--Columbia, 2019)
The use of somatic cell nuclear transfer (SCNT) to create agricultural and biomedical models is extremely useful for understanding how we can improve the efficiency of production agriculture, as well as how we can better understand the progression...
Glutamine metabolism and interactions with mechanistic target of rapamycin complex 1 in porcine preimplantation embryos
(University of Missouri--Columbia, 2021)
The ability to culture embryos has created opportunities for animal agriculture, including the refined selection of superior genetics, and for the medical field, allowing couples experiencing infertility to have biological children. Moreover...
Differential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryos
(University of Missouri--Columbia, 2010)
Nuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued ...
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
(University of Missouri--Columbia, 2010)
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos ...
The effects of elevated temperature on preimplantation-stage porcine embryos
(University of Missouri--Columbia, 2006)
It was the objective of these experiments to more fully characterize the biochemical and developmental responses of in vitro-produced preimplantation stage porcine embryos to elevated temperatures. In vitro fertilized (IVF) ...
Identification and quantification of differentally represented transcripts in preimplantation bovine embryos
(University of Missouri--Columbia, 2007)
Identification of transcripts present at key development stages in preimplantation embryos is critical for a better understanding of early embryogenesis. To that end, we characterized the relative abundance of multiple ...
Abundance of TRIM28, SETDB1, and TP53 mRNA is dynamically regulated during porcine early embryogenesis and is abnormal in preimplantation embryos produced by in vitro fertilization in comparison to in vivo derived and nuclear transfer derived embryos
(University of Missouri--Columbia, 2013)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] In vitro embryo production is important for research in animal reproduction, embryo transfer, transgenics, and cloning. However, in vitro-derived embryos ...
DNA methylation in the early porcine embryo
(University of Missouri--Columbia, 2007)
Reproductive technologies such as in vitro fertilization, intracytoplasmic sperm injection, parthenogenetic activation, and somatic cell nuclear transfer are powerful procedures in the production of animals for agriculture, basic research...
Improving viability of immunodeficient RAG2-/- pigs by non-specific porcine antibody administration
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Immunodeficient swine models such as the RAG2-/- model, are valuable to the field of regenerative medicine; however, their utility would greatly benefit ...