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The IUBMB enzyme nomenclature decision tree
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
This project attempts to map the decisions involved in categorizing an enzyme using the International Union of Biochemistry and Molecular Biology (IUBMB) enzyme nomenclature. The recommendations of the Nomenclature Committee of IUBMB...
Estrogen receptors in TRAMP C2 cells
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
and efficient method for detecting a specific protein among a mixture of many different ones. In conclusion, both estrogen receptor α and β are present in TRAMP C2 cells. With this confirmation, the cure to prostate cancer is one step closer because this TRAMP C...
Organization of the command system in the lamprey brain that initiates locomotor behavior
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
(DLM); and RS neurons (Paggett et al. 2004). Other experiments suggest the following organization for the command system: RLR VMD & DLM RS neurons à spinal CPGs In the present study, various lesions were made in the brain to interrupt some...
Analysis of the mtDNA insertion site on chromosome 9L in maize inbreds using fluorescence in situ hybridization
(University of Missouri--Columbia. Office of Undergraduate Research, 2008)
with recombination in this region of chromosome 9L. Using two mtDNA probes present in the insertions in both B73 and Mo17, we applied a chromosome painting technique called fluorescence in situ hybridization (FISH) to root-tip metaphase chromosomes and looked...
The effect of caloric restriction on the mitotic rates of mice
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
Caloric restriction has been shown to cause a number of biological changes in animals including the retardation of the aging process. Calorically- restricted animals show an average increase in life span of 30 - 50 %. In the present study we wanted...
Development of CAPS markers for Nicotiana
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
The area in which the ranges of Nicotiana longiflora and Nicotiana plumbaginifolia overlap presents an ideal system for studying the interaction and hybridization of a selfing and an outcrossing species. N. longiflora is characterized by long...
Oxidative stress alters cell morphology and cell death indices in immortalized astrocytes
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Menadione, usually known as vitamin K3, also serves as a trigger for oxidative stress, delivering reactive oxygen species (ROS) upon entering the cells. Astrocytes are glial cells that are found in the brain and are extremely ...
Aβ toxicity to SHSY-5Y human neuroblastoma cells
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that results in the loss of memory, language deterioration, confusion, restlessness, and eventually destroyed cognition. It is characterized by an ...
Exploring the functional decapping ability of the dogfish shark Nudt16 homolog
(University of Missouri--Columbia. Office of Undergraduate Research, 2008)
A nuclear decapping and binding protein, X29/Nudt16, was originally characterized in Xenopus, and has also been characterized in humans. It is suggested that not only the protein's sequence, but also its functions has been ...
The inhibition effect of phage display peptides on E. coli 0157:H7
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
E. coli 0157: H7 strain is a strong food borne pathogen that lives in the cattle GI tract. It has been estimated that 76 million or more food borne illness occurs in the United States every year. In the last few years, ...
Improving mouse-DMH screening capability by adding 2000 mouse CpG islands
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
DNA methylation alteration, in correlation with gene expression, is involved in development and progression of many cancers. Using a microarray based method, mouse-DMH (Differential Methylation Hybridization), our lab is ...
Phosphorylation of EGFR, ERK 1/2 and downstream transcription factors after P2Y2 receptor activation in a human submandibular gland cell line
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
P2 nucleotide receptors mediate a variety of biological responses and are activated by the extracellular nucleotides adenosine triphosphate (ATP), adenosine diphosphate (ADP), uridine triphosphate (UTP), uridine diphosphate ...
Determining the feasibility of anesthetization with a MS-222 solution in combination with cardiocentesis in male gray treefrogs (hyla versicolor)
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Ethyl 3-aminobenzoate (MS-222) is commonly used to anesthetize fish and amphibians, but few studies have investigated its effects on frogs. We experimented with the effects of combining the use of MS-222 with the extraction ...
Expression of a putative virulence protein of Haemophilus influenzae in E. coli
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Haemophilus influenzae, a small, gram-negative bacterium, is a commensal organism of the human upper respiratory system as well as an agent of diseases ranging from otitis media to meningitis. Nonencapsulated (nontypeable, ...
Retension of knobs in chromosome tips in maize
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
Knobs are deeply staining chromosomal sites on maize chromosomes. Molecularly, they are composed of a 180 base pair repeat. Their positions on the chromosomes are variable but usually internal in maize. In relatives, the ...
Mitochondrial DNA insertion into nuclear chromosomes of maize
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Every mitochondrion contains its own DNA separate from the nucleus. Over evolutionary time, most of the mitochondrial genes have moved to the nucleus so that now mitochondria require nuclear DNA to function. This type of ...
Investigating the relationship between repeated DNA and gene silencing in the model organism Neurospora crassa
(University of Missouri--Columbia. Office of Undergraduate Research, 2008)
In order to maintain genomic integrity, organisms must possess the capability to combat unwanted elements such as retroviruses and transposons. In the filamentous fungus Neurospora crassa, the expression from repeated ...
Characterization of gene and protein marker expression by human dental pulp stem cells
(University of Missouri--Columbia. Office of Undergraduate Research, 2008)
Neurodegenerative diseases result from deterioration of neurons or their myelin sheath that over time leads to brain dysfunction and premature death. Cells of the brain and spinal cord do not readily regenerate therefore ...
A phenotypic comparison of heterosis and inbreeding depression across different ploidy levels of maize
(University of Missouri--Columbia. Office of Undergraduate Research, 2008)
It is hypothesized that dosage-dependent gene regulators controlling the quantitative traits contribute to heterosis and inbreeding depression in maize. Heterosis, also known as hybrid vigor, refers to the phenomenon that ...
How caloric restriction extends life
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
People have always wanted to know how to live longer lives. Researchers have found that caloric restriction allows this in most animals. Caloric restriction (CR) is defined as the restriction of food but still enough to ...