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Nitrate and nitrite growth inhibition of Desulfovibrio strains
(University of Missouri--Columbia, 2014)
their positive effects and decrease their negative effects. Growth of the sulfate-reducing bacteria can be inhibited by the chemicals nitrate and nitrite. Nitrate and nitrite are also present in heavy-metal contaminated environments and limit the growth...
Plant protein lysine acetylation
(University of Missouri--Columbia, 2013)
on thousands of proteins from diverse species, yet as recently as 2011 was virtually unheard of in plant species. Presented herein are initial descriptions of the developing soybean seed lysine acetylome, and both the pea seedling mitochondrial proteome...
The role of Rex in regulation of sulfate reduction in Desulfovibrio vulgaris Hildenborough
(University of Missouri--Columbia, 2014)
Although the enzymes for dissimilatory sulfate reduction by microbes have been studied, the mechanism for transcriptional regulation of the encoding genes remain unknown. In the work presented here, the model sulfate-reducing microbe Desulfovibrio...
Bacterial ribosome conformational dynamics during translation termination and rescue
(University of Missouri--Columbia, 2017)
Hydrolysis of polypeptide from the ribosome is a critical step that must occur prior to the ribosome recycling phase of translation. Inability of cells to do so can result in translation inhibition and eventually leading ...
Protein components of the microbial mercury methylation pathway
(University of Missouri--Columbia, 2015)
fuels, and a more recent major escalation of widespread use of Hg in artisanal gold mining. The Hg cycle in the environment is complex and still not completely known. Among the many different forms of Hg present in the environment, Hg, mainly as Hg...
Comparison of standard treatments of breast cancer with new experimental treatments targeting caveolin-1
(University of Missouri--Columbia, 2013)
. The first chapter will present an overview of breast cancer and explain in more detail the subtypes of breast cancer, current treatments, and development of resistance. The more aggressive breast cancer subtypes are tamoxifen (TAM)-resistant and triple...
Elucidating the roles of the ATP7A copper transporter in cultured cells, tumor growth and metastasis
(University of Missouri--Columbia, 2013)
, connective tissue development, pigment formation, iron absorption and mobilization, integrity of the central nervous and immune systems. However, copper can also be toxic when present in excessive amounts, owing to its ability to facilitate reactive oxygen...
Improvement of Biochemistry 2002 through experimental modification and organization of course materials
(University of Missouri--Columbia, 2011)
The University of Missouri Biochemistry department offers a variety of courses, each designed with a specific purpose and scheduled to avoid overlap. Biochemistry 2002, sophomore lab, is currently an elective course meant to bridge the gap between...
Biophysical studies of protein dynamics
(University of Missouri--Columbia, 2014)
unwinding also can be observed when there is no additional factors present, which also confirms that ribosome itself is a helicase. Ribosome dynamics is the main theme of this dissertation. However, since I have worked on NMR dynamics for two years with Dr...
The role of the P2Y2 nucleotide receptor in salivary gland regeneration
(University of Missouri--Columbia, 2014)
, in response to injury or stress and is proposed to play important roles in the regeneration of a variety of tissues. The results presented in this dissertation indicate that P2Y2R activation with UTP enhances the migration, aggregation and self...
Interaction of secretory protein SecA with its binding partners : lipids and SecB
(University of Missouri--Columbia, 2016)
is trapped between the SecA and SecB in the complex, like a sandwich. Thus, transfer of the precursor presents an interesting topological problem. Nothing is known about the molecular details of the transfer of precursor along this pathway. In the project...
Modification of host and bacterial proteomes during host-pathogen interactions
(University of Missouri--Columbia, 2011)
-bacteria interactions at the protein level have only examined plant responses, and not bacterial responses. Furthermore, the plant responses were elicited with a single MAMP, instead of the entire bacterium. In the present studies, we used an Arabidopsis cell culture...
Mass spectrometry-based seed allergen quantification and applications in seed quality assessment
(University of Missouri--Columbia, 2012)
-derived food-products. New technologies (e.g. proteomics and mass spectrometry) can provide the means to compare allergen concentrations among seed varieties to evaluate the contribution of both genetic and environmental factors. The work presented, describes...
[alpha]A-crystallin derived peptide, [alpha]A66-80 induced aggregation and precipitation of soluble [alpha]-crystallin : a contribution to age-related cataract formation
(University of Missouri--Columbia, 2013)
accumulation of specific low molecular weight (LMW) peptides (<3.5-kDa) derived from crystallins. Prominent among LMW peptides is [alpha]A66-80, a peptide derived from [alpha]A-crystallin and present in increased concentrations in the water-insoluble fractions...
Targeting estrogen- and hedgehog-signaling pathways in prostate cancer
(University of Missouri--Columbia, 2009)
of the two estrogen receptors alpha (ERα) and beta (ERβ) in TRAMP mouse prostates switches upon progression from non-metastatic to metastatic tumor phenotype. During earlier stages of cancer, ERβ is the main estrogen receptor present, as the phenotype...
Structural interactions sites of matrix metalloproteinase-12 with collagen triple helix, micelles, and a natural product from green tea
(University of Missouri--Columbia, 2012)
was used to investigate the interaction between MMP-12 and a THP. This study aims to answer the question of how a THP binds to MMP-12. Preliminary structures of the complex presented here have converged. The THP binds to MMP-12 in a specific manner in one...
DotB : Legionella pneumophila's Dot/Icm Type IV Secretion System's AAA+ motor
(University of Missouri--Columbia, 2023)
The Gram-negative bacteria Legionella pneumophila is the causative agent of a severe form of pneumonia known as Legionnaire's Disease. Legionella utilizes its Defect in organelle trafficking/Intracellular multiplication ...
Characterizing the roles of an Arabidopsis Epsin n-terminal homology domain protein in plant innate immunity
(University of Missouri--Columbia, 2015)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The plasma membrane (PM) serves as a crucial point of contact between a plant cell and its environment. Localized at this critical interface are proteins ...
Divalent ion-binding and thermal stability studies on rat [beta]-parvalbumin and the evidence of influential distant amino acid residues affecting CD site ion affinity
(University of Missouri--Columbia, 2011)
Parvalbumins (PVs) are vertebrate-specific proteins (Mr 12,000), which harbor two EF-hand motifs known as the CD and EF sites. Although the CD and EF sites are typically high-affinity sites, the mammalian [beta]-PV exhibits ...
The use of FD-filamentous bacteriophage for the in vivo imaging of cancer
(University of Missouri--Columbia, 2006)
Improved diagnosis of cancer through targeted imaging of neoplasms is needed for early detection and treatment of cancer. We propose that the physical characteristics of filamentous bacteriophage (phage) are well suited ...