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Cocrystallization of pyrogallol[4]arenes with target molecules
(University of Missouri--Columbia, 2011)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Supramolecular chemistry, in its simplest and broadest description, is the chemistry of intermolecular interactions or chemistry of the noncovalent ...
Development of advanced chemometric methods for analysis of deep-ultraviolet resonance Raman and circular dichroism spectroscopic data for protein secondary structure determination
(University of Missouri--Columbia, 2015)
Determination of protein secondary structure has become an area of great importance in biochemistry and biophysics as protein secondary structure is directly related to protein function and protein related diseases. While NMR and x...
Development of advanced chemometric methods for the analysis of deep-UV resonance Raman spectra of proteins
(University of Missouri--Columbia, 2009)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Ultra violet resonance Raman (UVRR) is a powerful spectroscopic technique for determining the secondary structural content of proteins in solution. ...
The effects of structural modifications on sigma receptor binding
(University of Missouri--Columbia, 2007)
The sigma receptor is a unique receptor family that has two subtypes: sigma1 and sigma2. Sigma1 receptors are involved in several physiological functions such as psychosis and depression. Sigma2 receptors are expressed in ...
Novel insights into strand cleavage at an abasic site in DNA
(University of Missouri--Columbia, 2022)
Loss of a nucleobases from the deoxyribose backbone of DNA generates Abasic sites in DNA. Abasic sites are the most common unavoidable DNA damage lesion. It is important to understand the properties of Abasic site. The ...
Purification and characterization of A[beta] and probing the interactions between myricetin and A[beta] using various spectroscopic methods
(University of Missouri--Columbia, 2009)
spectra indicate that the environment around one or more of the aromatic residues is altered when myricetin is present. These findings suggest that both hydrophobic interaction and hydrophilic interaction play a role in the binding of myricetin with A[beta]....
Biomimetic reactions relevant to the study of abasic sites in DNA
(University of Missouri--Columbia, 2020)
Abasic (Ap) sites represent the most common form of DNA damage in mammalian cells. With steady state levels of 10,000 sites existing in each cell, there is a dire need to study these noxious biological electrophiles. While ...
Inactivation of protein tyrosine phosphatases by endogenous and dietary agents
(University of Missouri--Columbia, 2009)
Protein tyrosine phosphatases are a class of enzymes that control a number of critical signaling pathways inside cells. We have discovered a number of dietary and endogenous agents that are capable of modifying these ...
Formation and properties of endogenous adducts and crosslinks generated by amine-catalyzed strand cleavage at an AP site in duplex DNA
(University of Missouri--Columbia, 2022)
Abasic sites in DNA are a very common lesion. They are formed spontaneously and in the presence of biological amines, enzymes, and proteins. If unrepaired, abasic sites lead to DNA strand breaks and crosslinks. This thesis ...
Spectroscopic investigations of the beta-amyloid peptide
(University of Missouri--Columbia, 2008)
The focus of this project is two-fold: examining the native structures of three different fragments of the beta-amyloid (A[beta]) peptide, and attempting to overcome some of the difficulties encountered in such an examination. ...
Investigations into the chemistry of protein tyrosine phosphatase redox regulation
(University of Missouri--Columbia, 2009)
of inactivating PTPs through reversible oxidation of their active site cysteine thiolate may find use as therapeutic agents and/or tools for the study of diverse signal transduction pathways. In the body of work presented here we report the chemical properties...
Solvatochromic pyrogallarenes and pyrogallarene crystals
(University of Missouri--Columbia, 2018)
Pyrogallarenes have the ability to self-assemble into a variety of structures. Crystal forms ranging from bilayers to dimers and hexamers have been isolated. One unique cocrystal, PgC[subscript 3]-(CH[subscript 3])[subscript ...
Structural and kinetic studies of human aldh7a1 and aldh9a1
(University of Missouri--Columbia, 2021)
The regulation and detoxification of endogenously and exogenously derived aldehydes is paramount to cellular survival due to the highly reactive nature of aldehydes as electrophiles. Human aldehyde dehydrogenases (ALDHs) ...
Variable-temperature [superscript 1]H-NMR and AB initio study of 5-amino-imidazole-4-carboxamide (AICA) : competing paths for amide-H scrambling
(University of Missouri--Columbia, 2008)
chemistry and biochemistry are understood very well. In contrast, hypotheses regarding prebiotic nucleobase syntheses remain controversial. While such studies focused on aqueous solution chemistry for half a century, planetary nucleobase syntheses in frozen...
Biologically relevant chemistry of sulfur heterocycles : from redox regulation of PTP1B to the biological activity of s-deoxy leinamycin
(University of Missouri--Columbia, 2008)
-isothiazolidinone) from sulfenic acid at the active site of PTP1B. The mechanism for the formation of this novel sulfenyl amide is not clear. We employed 1,2-benzisothiazolidinone as chemical model to mimic the important functional groups present at the active site...
Diverse applications of covalent cross-links in duplex DNA: From anti-cancer drugs to the detection of disease-relevant single nucleotide polymorphisms to the synthesis of well-defined substrates for the study of novel DNA repair processes
(University of Missouri--Columbia, 2018)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Abasic (Ap) sites are a common form of DNA lesion that occur endogenously 50,000-200,000 per cell per day in mammals. The alkylation of the guanine and ...
Novel chemical mechanisms of DNA damage by natural products
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Leinamycin is a structurally novel natural product isolated from the Streptomyces with potent antitumor and antibiotic activity. In a thiol rich ...
Isotope harvesting from the beam dump cooling loop at the facility for rare isotope beams (FRIB)
(University of Missouri--Columbia, 2018)
Isotope harvesting from the beam dump cooling loop of the Facility for Rare Isotope Beams (FRIB) offers usable quantities of many isotopes unavailable anywhere else in the world. Many of these co-produced isotopes are of ...
Spectroscopic investigations of dendritic polymers as molecular containers
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Previous research has shown the fluorescent probe phenol blue will associate with the dendritic polymers polypropyleneimine (PPI) and polyamidoamine ...
Studies of natural products that reveal novel strategies for efficient modification of DNA
(University of Missouri--Columbia, 2006)
or anticancer therapeutics. Efficient DNA modifications by these agents usually endow them with outstanding biological efficiency. In the present dissertation, we investigated novel DNA damaging natural products with novel DNA modification strategies. We showed...