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Molecular basis of protein tyrosine phosphatase inhibition by biologically important small molecules with relevance to cell signaling pathways
(University of Missouri--Columbia, 2009)
Protein tyrosine phosphatase 1B (PTP1B) is an abundant mammalian enzyme and well known to be as a central player in the insulin and leptin signaling pathways. Peracetic acid is a strong oxidant molecule, endogenously ...
Spatially resolved and bulk zinc analysis in biological samples of patients at different stages of Alzheimer's disease by high resolution inductively coupled plasma mass spectrometry
(University of Missouri--Columbia, 2008)
One hypothesis that has gained considerable attention in the pathogenesis of Alzheimer's disease (AD) is trace element toxicity, and various elements, including zinc, have become the foci of renewed interest. The first ...
Radiotracers for examining biological functions of plants and microbes
(University of Missouri--Columbia, 2021)
Tracers are used for qualitative and quantitative investigation of a system. Radiotracers have a radionuclide to observe chemical or biological processes by detection of the radionuclide's decay energy. They are non-disruptive and non...
Development of a pH sensitive coumarin based chemosensor
(University of Missouri--Columbia, 2010)
Fluorescent probes have found several uses in monitoring the physiological state of cells. Recently, the Glass lab has developed a fluorescent sensor capable of binding norepinephrine in chromaffin cell vesicles. This ...
Inactivation of protein tyrosine phosphatases by endogenous and dietary agents
(University of Missouri--Columbia, 2009)
disrupting signaling pathways inside cells. It is possible that these small molecules are exerting their widely reported biological effect by the modification of protein tyrosine phosphatase activity. This information is critical to our understanding...
Determination of total selenium and seleno-amino acids in yeast and aquatic organisms by liquid chromatography and inductively coupled plasma mass spectrometry
(University of Missouri--Columbia, 2007)
-ICP-MS) was developed. Masses at 77, 78, 79, 81, and 82 were monitored and quantitation of Se was determined based on both 78Se and 82Se. SIDA-ICP-MS was successfully applied to the determination of total selenium in biological materials, such as selenized yeasts...
Ligands for the sigma receptor and the mu-opioid receptor
(University of Missouri--Columbia, 2007)
analogues were synthesized as sigma ligands. Sigma receptors binding assays were conducted for the piperidine analogues. The last chapter is concerned with the opioid receptors, which have three subtypes: mu, delta, and kappa. The [mu]-opioid receptor...
Biomimetic reactions relevant to the study of abasic sites in DNA
(University of Missouri--Columbia, 2020)
Abasic (Ap) sites represent the most common form of DNA damage in mammalian cells. With steady state levels of 10,000 sites existing in each cell, there is a dire need to study these noxious biological electrophiles. While much work has been done...
Structural, biochemical, and inhibition studies of proline biosynthetic enzymes
(University of Missouri--Columbia, 2019)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Pyrroline-5-carboxylate reductase (PYCR) is the final enzyme in proline biosynthesis, catalyzing the NAD(P)H-dependent reduction of [Delta]1-pyrroli ...
Evaluating the suitability of the human toenail as a biomonitor for manganese status: the one source cohort
(University of Missouri--Columbia, 2007)
Numerous studies have demonstrated that the human toenail is a reliable biomonitor for the intake of Se and other elements. The objective of this study was to evaluate the hypothesis that Mn intake is reflected in toenail ...
Biologically relevant chemistry of sulfur heterocycles : from redox regulation of PTP1B to the biological activity of s-deoxy leinamycin
(University of Missouri--Columbia, 2008)
results suggest that formation of sulfinyl amide may protect the enzyme against irreversible over-oxidation. In a different context, we examined possible chemical mechanisms underlying the biological activity of natural product S-deoxy leinamycin. We...
Measurement of radionuclides in biological tissues from occupationally exposed individuals
(University of Missouri--Columbia, 2022)
inductively coupled plasma mass spectrometry-based methods to measure the radionuclides 226Ra and 239Pu in brain tissue samples. This dissertation presents three methods to measure ultra-trace radionuclides in biological tissues. The methods involve chemical...
Investigations into the chemistry of protein tyrosine phosphatase redox regulation
(University of Missouri--Columbia, 2009)
Transmission of complex intracellular signals, such as those for glucose uptake or proliferation, is often accomplished through the reversible phosphorylation of specific protein tyrosine residues. This reversible ...
Synthesis of rhenium complexes as models for new sigma receptor imaging agents
(University of Missouri--Columbia, 2009)
The sigma receptor has been biologically found to play roles in the central nervous system (CNS) disorders and cancer cell proliferation. With the identification of lead sigma receptor binding ligands by previous structure activity relationship (SAR...
Development and application of inductively coupled plasma mass spectrometry techniques to investigate platinum chemotherapy agents
(University of Missouri--Columbia, 2010)
robust and highly sensitivity experimental quadrupole ICP-MS methodology was developed and used to analyze platinum and copper in biological matrices and acid-assistant closed-vessel microwave digestion and heated sonic water bath digestion were used...
Effects of local sequence on the formation of interstrand DNA cross-links derived from the reaction of an abasic site with a cytosine residue on the opposing strand
(University of Missouri--Columbia, 2019)
chemistry may result in increased biological relevance and advances in understanding this type of DNA damage....
Characterizing and understanding self-assembling, nanocapsule host-guest systems
(University of Missouri--Columbia, 2010)
Supramolecular, self-assembled nanocapsules have been shown to be capable of entrapping fluorescent guests. Previous solid- and solution-state research, focusing on hydrogen-bonded C-alkylpyrogallol[4]arenes (PgC6)nanocapsules, ...
pH sensitive fluorescent sensors
(University of Missouri--Columbia, 2007)
Pheochromocytoma is a cancer of the adrenal gland. Pheochromoocytomas produce a variety of catecholamines, including norepinephrine. The result is high blood pressure which is caused by the release of catecholamines into ...
Solvatochromic pyrogallarenes and pyrogallarene crystals
(University of Missouri--Columbia, 2018)
Pyrogallarenes have the ability to self-assemble into a variety of structures. Crystal forms ranging from bilayers to dimers and hexamers have been isolated. One unique cocrystal, PgC[subscript 3]-(CH[subscript 3])[subscript ...
Investigation of the replacement of cysteine residues in DOTA-(Tyr³)-octreotate: synthesis, characterization and evaluation of biological activities
(University of Missouri--Columbia, 2006)
Modification of the peptide sequence of DOTA-Tyr³-octreotate is carried out by substituting the cysteine moieties with D-Cysteine, L-Penicillamine and D-Penicillamine in one or both positions to study the effect of this ...