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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
(University of Missouri--Columbia, 2022)
of AAVR was not revealed in our previous structure, although it had been shown to be involved in cellular entry for many AAV serotypes, including AAV2. Utilizing cryogenic electron microscopy (cryo-EM) we explore the AAV5-AAVR interactions, using an AAV5...
Informatically-informed biochemical characterization of aptamer-protein complexes
(University of Missouri--Columbia, 2022)
Nucleic acid aptamers are single-stranded oligonucleotides that fold into unique three-dimensional shapes that typically bind to targets of interest with high specificity and high affinity. However, a significant limiting ...
Aptamers that bind filovirus GP : a new frontier for detection and neutralization of Ebola and Marburg virus
(University of Missouri--Columbia, 2020)
highly lytic vesicular stomatitis virus-based filovirus GP displayed surrogate viruses. Filoviruses are highly pathogenic and thus require highly secured containment facilities for their studies. The use of attenuated surrogates facilitates filovirus...
RNA structural studies : implications in HIV-1 replication and the origins of life
(University of Missouri--Columbia, 2021)
Structure and function relationships are important to understand many biological processes. Biological components are not one dimensional and their structures may drive key aspects of their function. This dissertation ...
Phage display peptides for breast cancer targeting
(University of Missouri--Columbia, 2010)
Cancer cell surfaces differ from healthy cell surfaces allowing the detection of cancer targets on the cell. Use of these targets combined with radiolabeled targeting vehicles results in sensitive imaging agents, with ...
Roles of dynamin-related protein network in plant immune responses and development
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT REQUEST OF AUTHOR.] At the host-pathogen interphase, the PM's protein composition has a critical role in how plants perceive and respond to the invading pathogenic ...
Skeletal muscle weakness and mitochondrial dysfunction in the osteogenesis imperfecta murine (oim) model
(University of Missouri--Columbia, 2021)
Osteogenesis imperfecta (OI), also commonly referred to as brittle bone disease, is a heritable connective tissue disorder occurring in roughly 1:15,000 births. OI arises as a result of mutations in the type I collagen ...
Pretargeted cancer radioimmunotherapy and radioimaging using antibodies and antibody fragments
(University of Missouri--Columbia, 2018)
Over the last few decades, antibodies have become the mainstay of cancer diagnosis and therapeutics. In traditional radioimmunotherapy (RIT), tumor targeting antibodies are directly conjugated with radioisotopes and depending ...
HIV-1 5'UTR RNA structures: connections to infectious lifecycle and potential therapeutic applications
(University of Missouri--Columbia, 2021)
relevance in the HIV-1 infectious lifecycle. In this dissertation, we map the binding interface of HIV-1 5'UTR TAR RNA with INI1, a core component of the SWI/SNF eukaryotic chromatin remodeling complex. This is the first example of HIV-1 RNA surface...
Amyloid beta induces cPLA2 activation by an NADPH oxidase-dependent mechanism in neurons
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Alzheimer's disease (AD) is a neurodegenerative disorder affecting more than 20 million people worldwide. An increase in production of amyloid beta ...
Glomerulosclerosis in the Col1a2-deficient mouse model : homotrimer pathogenesis and MMP expression
(University of Missouri--Columbia, 2009)
The Col1a2-deficient (oim) mouse model exclusively synthesizes homotrimeric type I collagen due to the lack of functional pro [alpha] 2(I) collagen chains. The mouse develops a type I collagen glomerulopathy that has ...