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Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation
(University of Missouri--Columbia, 2006)
outbreaks. Genomic DNA for the real-time PCR was extracted by the boiling method. Three sets of primers and corresponding TaqMan® probes were designed to target these three pathogens. Multiplex real-time PCR was carried out with TaqMan® Universal PCR Master...
Novel PCR-based rapid detection strategies for Escherichia coli O157:H7 and Salmonella in meat products
(University of Missouri--Columbia, 2009)
staining step, the detection range was 10₃ to 10₉ CFU/ml for pure cultures, 10₅ to 10₉ CFU/ml for artificially contaminated poult y samples, and 10₈ to 10₄ CFU/g for ground beef samples. After a 12-h enrichment step, EMA combined real-time PCR...