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Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster
(ACS, 2007)
is affected by phosphorylation. Using a proteomic approach, we have shown that protein kinase C (PKC) activity is necessary for mediating 20E-induced expression of 14 specific proteins, including three previously reported 20E responsive proteins, and is also...
Establishment of a protein reference map for soybean root hair cells
(American Society of Plant Biologists, 2008)
infection process, and also lead to improvements in plant water and nutrient uptake. We analyzed the proteome of isolated soybean (Glycine max) root hair cells using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and shotgun proteomics (1D...
Proteomics of canine lymphoma identifies potential cancer-specific protein markers
(American Association for Cancer Research, 2007)
: Thirteen lymph nodes from normal dogs and 11 lymph nodes from dogs with B-cell lymphoma were subjected to proteomic analysis using two-dimensional PAGE separation and matrix-assisted laser desorption/ionization time-of-flight analysis. Results: A total...
Cell wall proteome in the maize primary root elongation zone. II. Region-specific changes in water soluble and lightly ionically bound proteins under water deficit
(American Society of Plant Biologists, 2007)
comprehensive understanding of how cell wall protein (CWP) composition changes in association with the differential growth responses to water deficit in different regions of the elongation zone, a proteomics approach was used to examine water soluble and loosely...
Composition, vigor, and proteome of mature soybean seeds developed under high temperature
(Crop Society of America, 1999)
The effects of high temperature treatment on soybean [Glycine max (L.) Merr.] seed composition, vigor, and proteome were investigated using mature dry seeds harvested from plants grown in environment-controlled chambers. High day/night temperatures...
Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum
(APS Press, 2005-01)
, the functional genomics of a single, plant cell type. The results obtained also indicate that proteomic studies with soybean, lacking a complete genome sequence, are practical....
Bioinformatics methods for protein identification using peptide mass fingerprinting data
(University of Missouri--Columbia, 2009)
Protein identification using mass spectrometry is an important yet partially solved problem in the study of proteomics during the post-genomic era. The major techniques used in mass spectrometry are Peptide Mass Fingerprinting (PMF) and Tandem mass...
Effect of a single AGE modification on the structure and chaperone activity of human alphaB-Crystallin
(American Chemical Society, 2007)
During aging, human lens proteins undergo several post-translational modifications, one of which is glycation. This process leads to the formation of advanced glycation end products (AGEs) which accumulate with time possibly ...
Genetics and functional genomics of legume nodulation
(Elsevier, 2006-02)
Gram-negative soil bacteria (rhizobia) within the Rhizobiaceae phylogenetic family (a-proteobacteria) have the unique ability
to infect and establish a nitrogen-fixing symbiosis on the roots of leguminous plants. This ...
Maximal efficiency of coupling between ATP hydrolysis and translocation of polypeptides mediated by secB requires two protomers of secA
(American Society for Microbiology, 2008)
SecA is the ATPase that provides energy for translocation of precursor polypeptides through the SecYEG translocon in Escherichia coli during protein export. We have previously shown that when SecA receives the precursor ...
System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolism
(American Society of Plant Biologists, 2009)
Embryo-specific overexpression of biotin carboxyl carrier protein 2 (BCCP2) inhibited plastid acetyl-coenzyme A carboxylase (ACCase), resulting in altered oil, protein, and carbohydrate composition in mature Arabidopsis ...
Guest and ligand behavior in zinc-seamed pyrogallol[4]arene molecular capsules
(Wiley-VCH, 2007)
Not just an inside job: Encapsulated and confined in an octanuclear zinc-seamed pyrogallol[4]arene molecular capsule, a guest of choice can act as a reporter for electronic communication between the exterior and the interior ...
Development of a multi-omics approach to identify highly correlated transcriptomic, proteomic and metabolic signatures in maize B73 and FR697 drought stressed nodal roots
(University of Missouri--Columbia, 2021)
, we analyzed multiomics (transcriptome, proteome, metabolome) datasets generated from the growth zone of nodal roots collected from the reference inbred line B73 and from inbred line FR697, which exhibits a relatively greater ability to maintain root...
Drug resistance in D. discoideum: isolation of 4-nitroquinoline 1-oxide resistant mutants
(University of Missouri--Columbia, 2005)
The drug 4-nitroquinoline 1-oxide (4NQO) displays both carcinogenic and antitumor effects, a well known characteristic of many chemotherapeutic drugs. In addition 4NQO shares a similar operating mechanism with the commonly ...
Uncovering the genetic basis of seed amino acid composition in arabidopsis using a multi-omics integrative approach
(University of Missouri--Columbia, 2021)
to create a seed with a more balanced amino acid profile. However, upon perturbations to the proteome, mutant seeds have demonstrated a rebalancing phenomenon where even large alterations to the amino acid composition activate a compensation mechanism...
A custom oligonucleotide microarray analysis as a tool for dissecting soybean-bradyrhizobium japonicum nodule senescence
(University of Missouri--Columbia, 2007)
connecting the bacteroids. This study demonstrates how global analysis can lead to new and novel discoveries.--From public.pdf....
Peroxiredoxin 2 and peroxidase enzymatic activity of mammalian spermatozoa
(Society for the Study of Reproduction, 2009)
is reverted back to the reduced form by the thioredoxin system. In the present study, we investigated the presence of PRDX2 in mouse and boar spermatozoa as well as in mouse spermatids using proteomic techniques and immunocytochemistry. Sperm and spermatid...
Low-intensity blast-induced mild traumatic brain injury : linking blast physics to biological outcomes
(University of Missouri--Columbia, 2019)
of 350 g of high-energy explosive C4. The use of an open-field experimental blast generated a primary blast wave with a peak overpressure of 6.76 pounds per square inch (PSI) (46.6 kPa) at a 3-meter (m) distance from the center of the explosion...
Significance of interactions of low molecular weight crystallin fragments in lens aging and cataract formation
(The American Society for Biochemistry and Molecular Biology, 2008)
Analysis of aged and cataract lenses shows the presence of increased amounts of crystallin fragments in the high molecular weight aggregates of water-soluble and water-insoluble fractions. However, the significance of ...
Discovery and phenotyping of the determinants and receptors of post-fertilization sperm mitophagy
(University of Missouri--Columbia, 2023)
fertilization. In the context of fertilization events, a multi-pronged proteomic and sperm phenotyping approach is used to further understand the function and distribution of 185 candidate sperm mitophagy substrates and determinants identified by proteomic...