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Biophysical characterization of the structure and flexibility of the E. Coli ribosome
(University of Missouri--Columbia, 2019)
At the discovery of ribosomes by George Palade in 1955 in the first image of the subcellular environment, he described them as "a particulate component of small dimensions (100 to 150 [alpha]) and high density". Subsequently, ...
The influence of mRNA secondary structures on ribosome conformational dynamics
(University of Missouri--Columbia, 2016)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Synthesizing proteins in the cell is a critical aspect of life. Protein synthesis is a complicated process and involve highly functional machines at a ...
Stability, states, and interactions of the enzyme PMM/PGM by NMR and multivariate analysis
(University of Missouri--Columbia, 2016)
Phosphomannomutase/phosphoglucomutase (PMM/PGM) contributes to the infectivity of an opportunistic human pathogen Pseudomonas aeruginosa by participating in carbohydrate biosynthesis. As a phospho-transfer enzyme, PMM/PGM ...
Protein components of the microbial mercury methylation pathway
(University of Missouri--Columbia, 2015)
Mercury (Hg) in the environment and the resulting methylmercury (CH3Hg+) produced by microorganisms has emerged as a global concern in the last 50 years after a series of high profile, large scale contamination events, ...
Interaction of secretory protein SecA with its binding partners : lipids and SecB
(University of Missouri--Columbia, 2016)
Thirty percent of proteins synthesized in the cytoplasm of E.coli are exported across or into the cytoplasmic membrane to reach their final destinations. The general secretory system exports precursor proteins through the ...
Study of PVCA and PVCB, two enzymes involved in making isonitrile-containing natural products in bacterial pathogens
(University of Missouri--Columbia, 2016)
Isonitriles, with an unusual functionality, are very versatile intermediates widely involved in organic synthesis. Interestingly, a variety of isonitriles were encountered in both marine and terrestrial systems as the ...
Advancing single molecule fluorescence microscopy through the study of dynamic macromolecules
(University of Missouri--Columbia, 2017)
We took a two pronged approach to our investigation of protein synthesis by the ribosome: 1) the generation of a novel fluorescence enhancing substrates 2) the development of a method for the generation of fluorescent ...
Kinetic study of histidine kinase CheA in bacterial chemotaxis
(University of Missouri--Columbia, 2017)
Histidine kinase CheA is central to signaling in bacterial chemotaxis. This kinase is responsible for the phosphorylation of two response regulators, CheY and CheB. CheY controls flagellar rotation and thus motility. CheB ...
Informatically-informed biochemical characterization of aptamer-protein complexes
(University of Missouri--Columbia, 2022)
Nucleic acid aptamers are single-stranded oligonucleotides that fold into unique three-dimensional shapes that typically bind to targets of interest with high specificity and high affinity. However, a significant limiting ...
Structural studies of flavoprotein inhibitors and inactivators
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI--COLUMBIA AT REQUEST OF AUTHOR.] Flavoenzymes have a versatile capacity to catalyze a wide range of reactions, using their flavin cofactor. In this thesis, work on two ...
Role of Proa(2)I collagen chains and collagen crosslinking in thoracic aortic biochemical integrity during aging using the OIM mouse model
(University of Missouri--Columbia, 2006)
The extracellular matrix (ECM) is an important constituent for a variety of tissues including vascular tissue in which the ECM maintains aortic wall integrity. An important component of vascular tissue ECM is type I collagen. ...
DotB : Legionella pneumophila's Dot/Icm Type IV Secretion System's AAA+ motor
(University of Missouri--Columbia, 2023)
The Gram-negative bacteria Legionella pneumophila is the causative agent of a severe form of pneumonia known as Legionnaire's Disease. Legionella utilizes its Defect in organelle trafficking/Intracellular multiplication ...
A transcriptomic approach to uncovering the molecular mechanisms of maize nodal root growth maintenance during drought stress in the field
(University of Missouri--Columbia, 2021)
Drought is the most important factor limiting crop production across the globe. Therefore, understanding how plants adapt and survive drought conditions is critical for improving food production with lower water input. One ...
Investigation of the initial steps of jasmonic acid biosynthesis and a study of differential regulation of the ribosomal association of mRNA transcripts in jasmonate-dependent wound response
(University of Missouri--Columbia, 2020)
Jasmonic acid is an oxylipin derived hormone that is responsible for regulating plant responses against various abiotic and biotic stressors and it also serves important roles in plant development. Jasmonic acid once ...
Characterization of peptides and phage that bind galectin-3 selected from bacteriophage display libraries: a study of the role of galectin-3 in metastasis-associated cancer cell adhesion
(University of Missouri--Columbia, 2005)
Intravascular cancer cell adhesion plays a significant role in the metastatic process. Studies indicate that galectin-3, a member of the galectin family of soluble animal lectins, is involved in carbohydrate-mediated ...
Skeletal muscle weakness and mitochondrial dysfunction in the osteogenesis imperfecta murine (oim) model
(University of Missouri--Columbia, 2021)
Osteogenesis imperfecta (OI), also commonly referred to as brittle bone disease, is a heritable connective tissue disorder occurring in roughly 1:15,000 births. OI arises as a result of mutations in the type I collagen ...
Investigating changes in the Plasma Membrane proteome and role of USPA-related proteins during plant immune responses
(University of Missouri--Columbia, 2023)
[EMBARGOED UNTIL 5/1/2024] Food insecurity is a growing global concern as food prices continue to increase. While many factors influence the loss of food production, plant diseases are a contributing factor that result in ...
On the role of the purinergic receptor P2Y2R in oral cancer and salivary gland dysfunction
(University of Missouri--Columbia, 2023)
[EMBARGOED UNTIL 5/1/2024] Due to the important roles the oral cavity plays in our daily lives ranging from eating and drinking to speech, diseases of the oral cavity significantly impact the quality of life. Oral cancers ...
Targeted cancer radioimaging and radiotherapy and the development of tumor-avid molecules using phage display
(University of Missouri--Columbia, 2021)
Prostate cancer (PCa) is the second leading cause of cancer death in men and new biomarkers are needed to improve diagnostic and therapeutic approaches in aggressive forms of this disease. CD44v6, a splice variant of the ...
RNA structural studies : implications in HIV-1 replication and the origins of life
(University of Missouri--Columbia, 2021)
Structure and function relationships are important to understand many biological processes. Biological components are not one dimensional and their structures may drive key aspects of their function. This dissertation ...