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Bacterial ribosome conformational dynamics during translation termination and rescue
(University of Missouri--Columbia, 2017)
Hydrolysis of polypeptide from the ribosome is a critical step that must occur prior to the ribosome recycling phase of translation. Inability of cells to do so can result in translation inhibition and eventually leading ...
The musculoskeletal effect of exercise and soluble activin receptor type 2b in mouse models of osteogenesis imperfecta
(University of Missouri--Columbia, 2017)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Osteogenesis Imperfecta (OI) is a heritable connective tissue disorder primarily due to mutations in the type I collagen containing tissues, such as ...
Characterizing the roles of an Arabidopsis Epsin n-terminal homology domain protein in plant innate immunity
(University of Missouri--Columbia, 2015)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The plasma membrane (PM) serves as a crucial point of contact between a plant cell and its environment. Localized at this critical interface are proteins ...
The influence of mRNA secondary structures on ribosome conformational dynamics
(University of Missouri--Columbia, 2016)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Synthesizing proteins in the cell is a critical aspect of life. Protein synthesis is a complicated process and involve highly functional machines at a ...
Targeting estrogen- and hedgehog-signaling pathways in prostate cancer
(University of Missouri--Columbia, 2009)
Prostate cancer is the second most frequent cause of cancer deaths in men, and the risk to develop cancer increases with age. Since the lifespan of the society is progressively increasing, the importance of preventing the ...
Advancing single molecule fluorescence microscopy through the study of dynamic macromolecules
(University of Missouri--Columbia, 2017)
We took a two pronged approach to our investigation of protein synthesis by the ribosome: 1) the generation of a novel fluorescence enhancing substrates 2) the development of a method for the generation of fluorescent ...
Dissecting water stress-induced molecular responses in the maize primary root
(University of Missouri--Columbia, 2017)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Drought is an important environmental factor limiting crop growth and productivity. With additional pressures arising from population growth and global ...
Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
(University of Missouri--Columbia, 2022)
Adeno-associated virus is one of the leading delivery agents used in gene therapy today. While it is already used in the clinic, some aspects of its basic biology are still not yet fully understood. Only recently in 2017 ...
A novel role for the ATP7A copper transporter in cancer
(University of Missouri--Columbia, 2018)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Copper is a trace metal with a ready capacity to gain or donate electrons. This property is harnessed by numerous enzymes to perform vital functions ...
Investigating the roles and functions of plasma membrane E3 ligases and MKP1 in plant immunity
(University of Missouri--Columbia, 2021)
When a plant encounters a pathogen, a complex set of responses occurs on both sides of the interaction. In a process known as pattern-triggered immunity (PTI), the plant uses plasma membrane (PM)-localized proteins, or ...
Characterization of the effects of 3,3'-diindolylmethane (DIM) in the TRAMP mouse prostate cancer model and DIM's interaction with estrogen receptor signaling
(University of Missouri--Columbia, 2018)
3,3'- diindolylmethane (DIM) is an acid -derived compound formed during the digestion of indole-3-carbinol (I3C), which is one of the main compounds in cruciferous vegetables, such as broccoli, cabbage and kale. Studies ...
Roles of clathrin-coated vesicle networks in plant immunity and development
(University of Missouri--Columbia, 2018)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] For a plant cell, the plasma membrane (PM) is not only the interface with its environment but also a signaling platform. Proteins localized to the PM ...
Structural studies of flavoprotein inhibitors and inactivators
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI--COLUMBIA AT REQUEST OF AUTHOR.] Flavoenzymes have a versatile capacity to catalyze a wide range of reactions, using their flavin cofactor. In this thesis, work on two ...
Informatically-informed biochemical characterization of aptamer-protein complexes
(University of Missouri--Columbia, 2022)
Nucleic acid aptamers are single-stranded oligonucleotides that fold into unique three-dimensional shapes that typically bind to targets of interest with high specificity and high affinity. However, a significant limiting ...
Structural studies of domain of unknown function 507 (DUF507)
(University of Missouri--Columbia, 2023)
The crystal structure of the domain of unknown function family 507 protein from Aquifex aeolicus is reported (AaDUF507, UniProt O67633, 183 residues). The structure was determined in two space groups (C2221 and P3221) at ...
Single cell-type metabolomics of Medicago truncatula border cells and their roles in host-microbe interactions
(University of Missouri--Columbia, 2022)
Border cells are a specialized cell type surrounding the root apical meristematic region of many plant species, with the exception of the Brassicaceae family that possess characteristically different border-like cells. ...
Biophysical characterization of the structure and flexibility of the E. Coli ribosome
(University of Missouri--Columbia, 2019)
At the discovery of ribosomes by George Palade in 1955 in the first image of the subcellular environment, he described them as "a particulate component of small dimensions (100 to 150 [alpha]) and high density". Subsequently, ...
Effects of differential and combinatorial inhibition of myostatin and activin A in the G610 murine model of osteogenesis imperfecta
(University of Missouri--Columbia, 2021)
Bone fragility, increased susceptibility to fracture, and low bone mineral density (BMD) are hallmarks of osteogenesis imperfecta (OI), a rare skeletal disorder that arises primarily from defects in the generation, quantity ...
Roles of dynamin-related protein network in plant immune responses and development
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT REQUEST OF AUTHOR.] At the host-pathogen interphase, the PM's protein composition has a critical role in how plants perceive and respond to the invading pathogenic ...
HIV-1 5'UTR RNA structures: connections to infectious lifecycle and potential therapeutic applications
(University of Missouri--Columbia, 2021)
Human immunodeficiency virus (HIV) is among the most sinister pathogens plaguing man. As of now, we do not yet have a method to "cure" infection once the virus has integrated into the host genome. It has the highest mutation ...