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Now showing items 101-120 of 615
Protoemics of integral membrane proteins from developing Brassica napus
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
As plant seeds develop the accumulation of natural products, starch, oil, and protein undergo dramatic changes. At the early stages of seed filling in oilseeds starch is the principal component. Oil (triacylglycerol) and ...
Selective precipation of seed storage proteins in soybeans: Reducing the dynamic range of protein expression for in-depth proteome studies
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Seed storage proteins (SSPs) are a class of proteins which accumulate at high levels (up to 60% total protein) during plant seed development as a nitrogen reserve for germination. Due to the preponderance of SSPs, the ...
Fescue toxicosis affects the reproduction and thermoregulatory systems of male rats exposed to heat stress
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Tall fescue (Festuca arundinacia), a cool season forage grass, has been utilized by many farmers for over fifty year to feed livestock. Unfortunately, about half of fescue pastures are infected with an endophytic fungus ...
Subcellular localization of the Arabidopsis thaliana atDjC37 molecular chaperone protein
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
DjC37. It has been previously determined that atDjC6 is nuclear localized (Suo & Miernyk 2004 Protoplasma 224: 79-89). We now wish to determine the subcellular localization of atDjC37. In silico analysis of the atDjC37 deduced amino acid sequence (http...
Alternative transcripts of the RPS4 resistance gene in Arabidopsis: Do they produce truncated proteins?
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
alternative transcripts whose functions are not yet fully understood. The dominant RPS4 alternative transcripts are produced via alternative splicing causing the retention of either intron 3 or introns 2 and 3. Previous research revealed the combined presence...
Development of chick embryo explant cultures as an assay system to test mammalian stem cell migration and differentiation after transplantation
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Stem cells are undifferentiated cells capable of achieving multiple developmental fates. The embryonic stem (ES) cell can differentiate into all cells of the body. Our stem cell research focuses on the repair and/or renewal of cells in the central...
The use of primary dermal fibroblast cultures to evaluate type I collagen expression in the oim model mouse
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Osteogenesis imperfecta type III is a heritable disorder leading to impaired connective tissue function in type I collagen containing tissues including bone fragility, blue-grey sclera, short stature, and hearing loss. ...
Evidence that glutamate induced neurite retraction of reticulospinal neurons is dependent on calcium influx
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Locomotor systems of vertebrates consist of a command system in the brain that activates central pattern generators in the spinal cord to initiate locomotor behavior. Reticulospinal (RS) neurons are the output neural ...
Dietary genistein influences function of mouse aorta
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Cardiovascular disease is the leading cause of death for men and postmenopausal women in the United States. Estrogen is thought to be cardioprotective. An important action of estrogen is increasing endothelial-dependent ...
What role does phosphorylation of cardiac troponin I play in elevating cardiac contractility following adrenergic stimulation?
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Myocardial performance is enhanced when adrenergic receptors are stimulated by catecholamines. The enhanced performance is mediated by cAMP dependent protein kinase A (PKA), which phosphorylates several proteins within the ...
Terrestrial movements and foraging behavior of the gray treefrog during the breeding season
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Currently, conservation techniques aim to preserve metapopulation dynamics in order to prevent species' extinction. A metapopulation is composed of multiple populations that interact by the exchange of individuals. This ...
Investigation of type I collagen deposition in the glomeruli of COL1A2 deficient mice
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Type I collagen is the most abundant structural protein in the body, playing a major role in the strength and integrity of connective tissues. Alterations in the synthesis and structure of type I collagen result in a number ...
Development of an assay to measure cortisol using a standard glucose meter
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
When an animal is stressed many changes occur, such as altered behavior and reduced resistance to disease, and these effect population performance (Millspaugh 2004). Cortisol is a stress hormone produced by the adrenal glands to regulate...
Identification of chloroplast DNA insertions in nuclear chromosomes of maize B73 line using the FISH procedure
(University of Missouri--Columbia. Office of Undergraduate Research, 2005)
It is known that chloroplast DNA can incorporate itself into the nuclear genome of plants. However, the sites of chloroplast (ct) DNA integration into chromosomes of maize have not yet been analyzed. This project is the ...
Age and gender differences in the use of emotion words between adolescent friends
(University of Missouri--Columbia. Office of Undergraduate Research, 2008)
to express distress and affection, regardless of their gender. This fits with research indicating that friendships become increasingly intimate with age (Durell, 2004). These findings are discussed in terms of the socialization of gendered patterns...
Study of auxin responsive gene promoters in transient expression assays
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Transfection assays are an important tool for biochemists; the comparatively quick technique allows experimenters to see relative levels of expression of a particular gene in the presence or absence of a plant hormone ...
Effects of Cimicifuga racemosa on mammary gland development in mice [abstract]
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
would alter development of the mammary glands, we performed the following experiment. Thirty-two female, C57blackJ mice were obtained from the MU colony at 3-4 weeks of age. The mice were randomly assigned to either a control diet or the same diet...
Bovine oocyte permeability in 1, 2-propanediol
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Cryopreservation of oocytes is of great importance both in medical research and in the agricultural industry. Cryopreservation of oocytes allows for future breeding of selected genetic lines of animals. Effective cryopreservation of bovine oocytes...
Kinetics vs. equilibrium binding: Determining the mechanism of transcriptional activation of Polymerase II
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
research, the equilibrium binding constants of wild-type TFIIIA-VP16 and two mutants, L148F and L148H, were determined, along with the half lives of the DNA-protein binding complexes containing these proteins. It was found that the equilibrium constants...
Leptin concentration in mares and their subsequent offspring [abstract]
(University of Missouri--Columbia. Office of Undergraduate Research, 2004)
Leptin is an adipocyte-derived hormone that influences an animal's feed intake and immune response, as well as body temperature regulation in the neonate. Additionally, leptin serves as an indicator of total body fat mass ...