Electron Microscopy Core Facility publications (MU)https://hdl.handle.net/10355/37642024-03-28T09:44:38Z2024-03-28T09:44:38ZDisseminated chlorellosis in a dogQuigley, R. R.Knowles, K.E.Johnson, G. C.https://hdl.handle.net/10355/37662020-06-23T19:47:17Z2009-01-01T00:00:00ZDisseminated chlorellosis in a dog
Quigley, R. R.; Knowles, K.E.; Johnson, G. C.
An adult dog with ataxia and a lingual mass, previously diagnosed as protothecosis, was euthanized. At the postmortem examination, the lingual mass, regions of the lungs and hilar lymph nodes, liver, mesenteric and sublumbar lymph nodes, and spinal meninges had pronounced green discoloration. Histologically, pyogranulomatous inflammation and algal organisms were found in the tongue, spinal meninges, hilar and mesenteric lymph nodes, liver, and lung. The algae had cell walls positive for periodic acid-Schiff and cytoplasmic granules. Ultrastructurally, the algae had a well-defined cell wall, stacks of grana and thylakoid membrane, and dense bodies, typical of starch granules. The organisms were identified as Chlorella, a green alga, based on the results of histochemistical and electron microscopic examination. To the author's knowledge this is the first report of disseminated Chlorella infection and the first report in a companion animal.
2009-01-01T00:00:00ZElectrochemical properties of carbon nanoparticles entrapped in silica matrixBok, Sangho, 1972-Lubguban, Arnold A.Gao, YuanfangBhattacharya, Shantanu, 1974-Korampally, Venumadhav, 1972-Hossain, MarufGangopadhyay, Shubhrahttps://hdl.handle.net/10355/39042020-06-23T19:47:17Z2008-01-01T00:00:00ZElectrochemical properties of carbon nanoparticles entrapped in silica matrix
Bok, Sangho, 1972-; Lubguban, Arnold A.; Gao, Yuanfang; Bhattacharya, Shantanu, 1974-; Korampally, Venumadhav, 1972-; Hossain, Maruf; Gangopadhyay, Shubhra
Carbon-based electrode materials have been widely used for many years for electrochemical charge storage, energy generation, and catalysis. We have developed an electrode material with high specific capacitance by entrapping graphite nanoparticles into a sol gel network. Films from the resulting colloidal suspensions were highly porous due to the removal of the entrapped organic solvents from sol-gel matrix giving rise to high Brunauer-Emmett-Teller (BET) specific surface areas (654 m2/g)and a high capacitance density (∼37 F/g). An exponential increase of capacitance was observed with decreasing scan rates in cyclic voltammetry studies on these films suggesting the presence of pores ranging from micro (< 2 nm) to mesopores. BET surface analysis and scanning electron microscope images of these films also confirmed the presence of the micropores as well as mesopores. A steep drop in the double layer capacitance with polar electrolytes was observed when the films were rendered hydrophilic upon exposure to a mild oxygen plasma. We propose a model whereby the microporous hydrophobic sol-gel matrix perturbs the hydration of ions which moves ions closer to the graphite nanoparticles and consequently increase the capacitance of the film.
2008-01-01T00:00:00ZGreen nanotechnology from cumin phytochemicals : generation of biocompatible gold nanoparticlesKatti, Kavita K.Chanda, NripenShukla, RaviZambre, AjitSuibramanian, ThilakavathiKulkarni, Rajesh R.Kannan, RaghuramanKatti, Kattesh V.https://hdl.handle.net/10355/37612020-06-23T19:50:50Z2009-01-01T00:00:00ZGreen nanotechnology from cumin phytochemicals : generation of biocompatible gold nanoparticles
Katti, Kavita K.; Chanda, Nripen; Shukla, Ravi; Zambre, Ajit; Suibramanian, Thilakavathi; Kulkarni, Rajesh R.; Kannan, Raghuraman; Katti, Kattesh V.
The powerful antioxidant characteristics of various phytochernicals within cumin prompted us to test their efficacy in reducing sodium tetrachloroaurate to corresponding gold nanoparticles. We, herein, report an unprecedented synthetic route that involves the production of well-defined spherical gold nanoparticles by simple mixing of cumin to an aqueous solution of sodium tetrachloro aurate. Production of gold nanoparticles in this cumin-mediated Green Nanotechnological process is achieved under biologically benign conditions. The gold nanoparticles generated through cumin-mediated process did not aggregate suggesting that the cocktail of phytochemicals including proteins serve as excellent coatings on nanoparticles and thus, provide robust shielding from aggregations. In addition, the phytochemical coatings on nanoparticles have rendered nontoxic features to these 'Green Gold Nanoparticles' as demonstrated through detailed MTT assays performed on 'normal fibroblast cells. Results of our studies presenting a new 'Nano-Naturo' connection for the production and utility of gold nanoparticles for potential applications in nanomedicine and nanotechnology are discussed in this paper.
Published in final edited form as:
Int J Green Nanotechnol Biomed. 2009 January 1; 1(1): B39-B52. doi:10.1080/19430850902931599.
2009-01-01T00:00:00ZGreen nanotechnology from tea : phytochemicals in tea as building blocks for production of biocompatible gold nanoparticlesNune, Satish K.Chanda, NripenShukla, RaviKatti, Kavita K.Kulkarni, Rajesh R.Thilakavathy, SubramanianMekapothula, SwapnaKannan, RaghuramanKatti, Kattesh V.https://hdl.handle.net/10355/37652020-06-23T19:47:17Z2009-01-01T00:00:00ZGreen nanotechnology from tea : phytochemicals in tea as building blocks for production of biocompatible gold nanoparticles
Nune, Satish K.; Chanda, Nripen; Shukla, Ravi; Katti, Kavita K.; Kulkarni, Rajesh R.; Thilakavathy, Subramanian; Mekapothula, Swapna; Kannan, Raghuraman; Katti, Kattesh V.
Phytochemicals occluded in tea have been extensively used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Results on the reduction capabilities of phytochemicals present in tea to reduce gold salts to the corresponding gold nanoparticles are presented in this paper. The phytochemicals present in tea serve a dual role as effective reducing agents to reduce gold and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. The tea-generated gold nanoparticles (T-AuNPs), have demonstrated remarkable in vitro stability in various buffers including saline, histidine, HSA, and cysteine solutions. T-AuNPs with phytochemical coatings have shown significant affinity toward prostate (PC-3) and breast (MCF-7) cancer cells. Results on the cellular internalization of T-AuNPs through endocytosis into the PC-3 and MCF-7 cells are presented. The generation of T-AuNPs follows all principles of green chemistry and T-AuNPs have been found to be non toxic as assessed through MTT assays. No 'man made' chemicals, other than gold salts, are used in this truly biogenic, green nanotechnological process thus paving the way for excellent opportunities for their application in molecular imaging and therapy.
2009-01-01T00:00:00Z