Dentistry Electronic Theses and Dissertations (UMKC)The items in this collection are the scholarly output of UMKC graduate students.https://hdl.handle.net/10355/99092024-03-29T15:24:03Z2024-03-29T15:24:03ZA Family Affected By Autosomal Dominant Non-Syndromic Tooth Agenesis - A Genetic StudySchlosser, Collin M.https://hdl.handle.net/10355/789032020-12-04T15:23:06Z2020-01-01T00:00:00ZA Family Affected By Autosomal Dominant Non-Syndromic Tooth Agenesis - A Genetic Study
Schlosser, Collin M.
This study evaluated a family exhibiting the phenotype of non-syndromic tooth agenesis through whole exome sequencing to potentially identify pathogenic variants. Four family members, three with tooth agenesis and one without, were enrolled as on-site subjects for whole exome sequencing analysis using genomic DNA isolated from their blood samples. Variants found to segregate with the tooth agenesis phenotype were evaluated further. An additional three family members, all with tooth agenesis, were enrolled as off-site subjects. DNA isolated from these subjects were evaluated by means of polymerase chain reaction and restriction enzyme testing to confirm or exclude the potential candidate variants based on their segregation in the off-site subjects.
Twenty-six variants were found to segregate with the tooth agenesis phenotype among the four on-site subjects. Potentially pathogenic mutants were identified through data filtering techniques such as DOMINO, CADD, and pLI. Of the twenty-six variants, seven heterozygous variants in novel candidate genes for tooth agenesis were identified: AHCYL1, WNT5B, HOXB2, VMP1, JUP, RNF43, and TP1. Only two variants created or destroyed a restriction enzyme site and thus, were able to be evaluated in the off-site subjects utilizing PCR/restriction enzyme testing, resulting in the elimination of JUP as a candidate variant.
The six remaining novel candidate genes were then assessed through a literature review. It was determined that WNT5B, HOXB2, and RNF43 may have a role in early odontogenesis by potentially altering the key dental epithelium/mesenchyme interactions. RNF43 emerged as the most likely causal variant due to the significance of the altered interaction site between RNF43’s binding groove and RSPO1’s β-hairpin protrusion, ultimately leading to dysregulation of the Wnt signaling pathway. Also, it was revealed that three families, in a larger genetic study evaluating tooth agenesis, had mutations in RNF43. One of which, had an identical change at the 97th position from an aspartic acid to an asparagine, which further strengthened our belief in RNF43 being the causal variant. Analysis of the un-recruited family members and selective sequencing of the off-site subjects for the five candidate variants that did not alter restriction enzyme sites could help to rule out oligogenic inheritance.
Title from PDF of title page viewed December 4, 2020; Thesis advisor: Mark Johnson; Vita; Includes bibliographical references (pages 52-60); Thesis (M.S.)--School of Dentistry. University of Missouri--Kansas City, 2020
2020-01-01T00:00:00ZA universal approach to stabilize water-oil interface via surfactant self-assemblyHonaryar, Houmanhttps://hdl.handle.net/10355/978212024-01-12T21:34:21Z2023-01-01T00:00:00ZA universal approach to stabilize water-oil interface via surfactant self-assembly
Honaryar, Houman
Liquid-liquid interfaces represent an advantageous setting for the confinement, manipulation, and controlled assembly of colloids. Stabilizing liquid-liquid interfaces, whether between miscible or immiscible liquids, is crucial for a wide range of applications, including energy storage, microreactors, and biomimetic structures. While most of the attention has been on the adsorption of colloidal or surface-active particles and polymeric complexion, there have been no studies concerning the self-assembly of small molecules like surfactants. Therefore, in this study:
First, a novel liquid-in-liquid 3D printing approach is developed to successfully shape extremely soft materials into complex and mechanically robust constructs with internal nanostructures using in situ self-assembly of surfactants. Second, using experimental techniques (visual inspection, small-angle X-ray scattering, rheological measurements, and microscopy) and a simulation technique (dissipative particle dynamics), the ternary phase diagram for the same material system (involving water, surfactant, and polar oil) that was used in liquid-in-liquid 3D printing is established to study the equilibrium phase behavior. The ternary phase diagram obtained from the simulations agrees with the experimental results, specifically in terms of morphological transitions, indicating the robustness of the computational simulation as a supplement to the mesoscale experimental systems. Third, an exhaustive and comprehensive overview of the state-of-the-art research on liquid-in-liquid 3D printing techniques is provided, explaining their fundamental principles, underlying mechanisms, and various material systems. Moreover, the practical features in these 3D printing platforms such as structural, mechanical, optical, magnetic, and communicative properties of prints along with the future potential of the technology and its limitations are discussed in detail. Lastly, the universality and versatility of the use of surfactant self-assembly for stabilizing the water-oil interface are studied for a wide range of surfactant classes and the underlying morphological transition was studied in equilibrium and dynamics conditions using a complementary combination of experimental and computational methods.
Title from PDF of title page, viewed January 12, 2024; Dissertation advisor: Zahra Niroobakhsh; Vita; Includes bibliographical references (pages 197-231); Dissertation (Ph.D.)--Department of Civil and Mechanical Engineering, Department of Oral Biology and Craniofacial Sciences. University of Missouri--Kansas City, 2023
2023-01-01T00:00:00ZAcetaminophen Versus Ibuprofen for the Control of Immediate and Delayed Pain Following Orthodontic Separator PlacementKawamoto, Shelliann A.https://hdl.handle.net/10355/89972020-09-10T10:37:14Z2010-01-01T00:00:00ZAcetaminophen Versus Ibuprofen for the Control of Immediate and Delayed Pain Following Orthodontic Separator Placement
Kawamoto, Shelliann A.
The purpose of this investigation was to compare pain control effectiveness of preemptive and postoperative acetaminophen, ibuprofen, and placebo following orthodontic separator placement. Subjects were randomly assigned to 1 of 3 conditions: placebo, 650 mg acetaminophen, or 400 mg ibuprofen. The placebo or analgesic was taken 1 hour prior to separator placement and 6 hours thereafter. Pain on chewing, teeth touching and biting was recorded on Visual Analogue Scales at 6 time intervals over 24 hours. Pain increased immediately after separator placement, decreased at 2 hours, then increased with variation into the next day. While preemptive analgesic decreased initial pain levels, no similar benefit was found from the postoperative dose. Differences between the two analgesics were not statistically significant, and no more effective than the placebo. Preemptive acetaminophen and ibuprofen are equally effective in controlling early pain following separator placement.
Thesis advisor: Karen B. Williams, R.D.H., M.S., Ph.D; Title from PDF of title page, viewed on November 9, 2010.; Vita.; Includes bibliographic references (pages 50-53).; Thesis (M.S.)--School of Dentistry. University of Missouri--Kansas City, 2010.
2010-01-01T00:00:00ZAdvanced Education Orthodontics Program Curricula Pre- and Post-COVID-19Grady, Patrickhttps://hdl.handle.net/10355/877222021-11-29T15:01:41Z2021-01-01T00:00:00ZAdvanced Education Orthodontics Program Curricula Pre- and Post-COVID-19
Grady, Patrick
A survey was conducted to investigate faculty numbers across advanced education
orthodontic programs and its association with curriculum, didactic learning, utilization of
distance education, and impact of the COVID-19 interruption. An 18-item survey, approved
by the UMKC IRB, was developed and sent to 65 program directors of United States
advanced education orthodontic programs. The survey was divided into the following four
domains: program description, curriculum organization, faculty numbers, and distance
education. No significant relationships were found regarding faculty numbers and curriculum
structure. No significant relationships were found concerning distance education or the
COVID-19 interruption. Interesting findings included structured didactic teaching levels
being higher than that proposed by the World Federation of Orthodontists. A trend seen in
this research consistent with previous literature was a decrease in curriculum time allocated
to research, while a deviation from the trend was an increase in full/half-time faculty
members. In summary, with the recent adoption of accreditation changes by the Commission
on Dental Accreditation regarding increasing faculty number requirements, another study
could assess this impact and continue to discover the role the current faculty play in how
curriculums are evolving.
Title from PDF of title page viewed November 8, 2021; Vita; Includes bibliographical references (pages 27-29); Thesis (M.S.)--School of Dentistry. University of Missouri--Kansas City, 2021; Thesis advisors: Mary P. Walker and JoAnna M. Scott
2021-01-01T00:00:00Z