Journal of Interdisciplinary Research (JIDR), vol. 1, no. 1 (2007)
https://hdl.handle.net/10355/10066
The items in this collection are the scholarly output of doctoral UMKC students .2024-03-29T11:28:28ZBoth P-gp and MRP2 mediate transport of Lopinavir, a protease inhibitor
https://hdl.handle.net/10355/10073
Both P-gp and MRP2 mediate transport of Lopinavir, a protease inhibitor
Agarwal, Sheetal; Pal, Dhananjay; Mitra, Ashim K.
Polarized epithelial non-human (canine) cell lines stably transfected with human or murine complementary DNA (cDNA) encoding for various efflux transporters (P-gp/MDR1, MRP1, MRP2, and Bcrp1) were used to study transepithelial transport of Lopinavir (LVR) and compare results with the MDCKII-Wild type cells. These transmembrane proteins cause multidrug resistance by decreasing the total intracellular accumulation of drugs. Lopinavir efflux was directional and was completely inhibited by MK-571, a selective MRP family inhibitor in the MDCKII-MRP2 cell line. Similarly, LVR efflux was also inhibited by P-gp inhibitors P-gp 4008 and GF120918 in
the MDCKII-MDR1 cell line. The efflux ratios (Efflux rate/ Influx rate) of LVR in the absence of any efflux inhibitors in the MDCK-Wild type, MDCKII-MDR1, MDCKII-MRP1, MDCKII-MRP2 and MDCKII-Bcrp1 cell monolayers were 1.32, 4.91, 1.26 and 2.89 respectively. The MDCKII-MDR1 and MDCKII-MRP2 cells have significantly increased LVR efflux ratio relative to the parental cells due to the apically directed transport by MDR1 and MRP2 respectively. The efflux ratios in MRP2 and MDR1 transfected cell lines were close to unity in the presence of MK-571 and P-gp 4008 respectively; indicating that LVR efflux by MRP2 and P-gp was completely inhibited by their selective inhibitors. MDCKII-MRP1 cells did not exhibit a significant reduction in the LVR efflux relative to the parental cells, indicating that LVR is not a good substrate for MRP1. Transport studies across MDCKII-Bcrp1 cells indicated that LVR is not transported by Bcrp1 and is not a substrate for this efflux protein. In
conclusion, this study presents direct evidence that LVR is effluxed by both P-gp and MRP2 which may contribute to its poor oral bioavailability and limited penetration into the CNS.
2007-01-01T00:00:00ZDiverse Struggles to Preserve Tribal Identity on the Plains: Religion as Survival Strategy in the Late Nineteenth Century among the Lakota and Osage
https://hdl.handle.net/10355/10079
Diverse Struggles to Preserve Tribal Identity on the Plains: Religion as Survival Strategy in the Late Nineteenth Century among the Lakota and Osage
Riggs, Michael S.
2007-01-01T00:00:00ZGoethe's Plant Morphology: The Seeds of Evolution
https://hdl.handle.net/10355/10078
Goethe's Plant Morphology: The Seeds of Evolution
Kelley, Tanya
It has long been debated whether
the scientific writing of Johann
Wolfgang von Goethe (1749-1832)
provided the seeds for the theory of
evolution. Scholars have argued both
sides with equal passion. German
biologist and philosopher, Ernst Haeckel
(1834-1919) wrote, “Jean and Lamarck
and Wolfgang Goethe stand at the head
of all the great philosophers of nature
who first established a theory of organic
development, and who are the illustrious
fellow workers of Darwin.”1 Taking the
opposite stance was Chancellor of Berlin
University, Emil du Bois Reymond
(1818-1896). Du Bois was embarrassed
by Goethe's forays into science. He
wrote, “Beside the poet, the scientist
Goethe fades into the background. Let
us at long last put him to rest.”2 I argue
that Goethe's scientific writings carry in
them the seeds of the theory of evolution.
Goethe's works on plant morphology
reflects the conflicting ideas of his era on
the discreteness and on the stability of
species. Goethe's theory of plant
morphology provides a link between the
discontinuous view of nature...
2007-01-01T00:00:00ZHeterodox Microfoundations: A Methodological Appraisal
https://hdl.handle.net/10355/10076
Heterodox Microfoundations: A Methodological Appraisal
Jo, Tae-Hee
This paper examines underlying methodological commitments in orthodox and heterodox approaches to micro and macro. Identifying methodological advantages and drawbacks of existing microfoundations, macrofoundations,
and mesofoundations, I uphold the need for the heterodox microfoundations of macroeconomics which is centered on the casual mechanisms of an economic system. By this, we are capable of analyzing micro- and macroreality which are recursively interrelated. In addition, the fallacy of macroreductionism and central-reductionism can be avoided. One way of
articulating the heterodox microfoundations is to utilize a circular production input-output matrix of a capitalist system combined with the price system. Such a framework is consistent with heterodox economics at the methodological and theoretical level.
2007-01-01T00:00:00Z