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dc.contributor.advisorNguyen, Henry T.en_US
dc.contributor.authorTran, Huong Nguyen Thanh, 1983-en_US
dc.date.issued2009eng
dc.date.submitted2009 Fallen_US
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on February 22, 2011).en_US
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.en_US
dc.descriptionThesis advisor: Dr. Henry T. Nguyen.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionM. S. University of Missouri--Columbia 2010.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Agronomy.en_US
dc.description.abstractDrought is the major abiotic stress factor limiting crop productivity worldwide. Plant root and shoot systems respond to environmental changes by altering the expression of complex gene networks through sensing environmental stresses and modifying signaling and metabolic pathways. Previous work (Yamaguchi et al., 2009) showed that the soybean primary root adapts to low water potential (-1.6 MPa) by maintaining longitudinal expansion in the apical 4 mm (region 1), whereas in the adjacent 4 mm (region 2), longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low water potential. To identify the key transcription factors (TFs) that determine these responses to low water potential, we have conducted high-throughput profiling of root-related TF expression in regions 1 and 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after transplanting. Several stress-specific and root-region-specific transcripts were identified which may contribute to root responses to water deficits. Among these were zinc-finger protein, MYB-related protein, GmNAC3, GmNAC4, and bZIP transcription factors. These TFs were differentially expressed in distinct root regions, and therefore they can be targeted for functional characterization and further genetic engineering for enhanced drought resistance in soybean.en_US
dc.format.extentxii, 100 pagesen_US
dc.identifier.oclc704275412en_US
dc.identifier.otherTranH-121109-T807en_US
dc.identifier.urihttp://hdl.handle.net/10355/10129
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2009 Freely available theses (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2009 Theses
dc.subject.lcshSoybean -- Roots -- Growthen_US
dc.subject.lcshSoybean -- Drought toleranceen_US
dc.subject.lcshTranscription factorsen_US
dc.subject.lcshDeficit irrigationen_US
dc.titleHigh throughput profiling of transcription factors involved in soybean root growth under water deficiten_US
dc.typeThesisen_US
thesis.degree.disciplineAgronomyeng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMastersen_US
thesis.degree.nameM.S.en_US


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