High throughput profiling of transcription factors involved in soybean root growth under water deficit

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High throughput profiling of transcription factors involved in soybean root growth under water deficit

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/10129

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dc.contributor.advisor Nguyen, Henry T. en_US
dc.contributor.author Tran, Huong Nguyen Thanh, 1983- en_US
dc.date.accessioned 2011-03-04T15:11:52Z
dc.date.available 2011-03-04T15:11:52Z
dc.date.issued 2009 en_US
dc.date.submitted 2009 Fall en_US
dc.identifier.other TranH-121109-T807 en_US
dc.identifier.uri http://hdl.handle.net/10355/10129
dc.description Title from PDF of title page (University of Missouri--Columbia, viewed on February 22, 2011). en_US
dc.description The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. en_US
dc.description Thesis advisor: Dr. Henry T. Nguyen. en_US
dc.description Includes bibliographical references. en_US
dc.description M. S. University of Missouri--Columbia 2010. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Agronomy. en_US
dc.description.abstract Drought is the major abiotic stress factor limiting crop productivity worldwide. Plant root and shoot systems respond to environmental changes by altering the expression of complex gene networks through sensing environmental stresses and modifying signaling and metabolic pathways. Previous work (Yamaguchi et al., 2009) showed that the soybean primary root adapts to low water potential (-1.6 MPa) by maintaining longitudinal expansion in the apical 4 mm (region 1), whereas in the adjacent 4 mm (region 2), longitudinal expansion reaches a maximum in well-watered roots but is progressively inhibited at low water potential. To identify the key transcription factors (TFs) that determine these responses to low water potential, we have conducted high-throughput profiling of root-related TF expression in regions 1 and 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after transplanting. Several stress-specific and root-region-specific transcripts were identified which may contribute to root responses to water deficits. Among these were zinc-finger protein, MYB-related protein, GmNAC3, GmNAC4, and bZIP transcription factors. These TFs were differentially expressed in distinct root regions, and therefore they can be targeted for functional characterization and further genetic engineering for enhanced drought resistance in soybean. en_US
dc.format.extent xii, 100 pages en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject.lcsh Soybean -- Roots -- Growth en_US
dc.subject.lcsh Soybean -- Drought tolerance en_US
dc.subject.lcsh Transcription factors en_US
dc.subject.lcsh Deficit irrigation en_US
dc.title High throughput profiling of transcription factors involved in soybean root growth under water deficit en_US
dc.type Thesis en_US
thesis.degree.discipline Agronomy en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name M.S. en_US
thesis.degree.level Masters en_US
dc.identifier.oclc 704275412 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2009 Theses
dc.relation.ispartofcollection 2009 Freely available theses (MU)


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