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dc.contributor.advisorFan, Xudongeng
dc.contributor.authorSuter, Jonathan Daniel, 1982-eng
dc.date.issued2010eng
dc.date.submitted2010 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on December 7, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionDissertation advisor: Dr. Xudong Fan.eng
dc.descriptionVita.eng
dc.descriptionPh. D. University of Missouri--Columbia 2010.eng
dc.description.abstractFor several decades, optical ring resonators have played an important role in the field of telecommunications. Within only the past decade however, ring resonators have been applied for the purposes of biological and chemical detection by means of refractometric signal transduction. The advantage of ring resonator-based sensing is that, unlike fluorescence detection, it is label-free and can yield quantitative data. This document presents the fundamental operation of the versatile opto-fluidic ring resonator (OFRR) and explores its applications in DNA methylation analysis. Using affinity assays based on anti-5-methyl cytosine antibodies as well as the methyl binding protein MBD-2, it is shown that the OFRR is a promising tool for biomedical research that can discriminate the extent of DNA methylation. As a part of evolving this and other devices towards a practical laboratory and clinical tool, it is important to consider methods for integrating optical functionality onto a compact chip. Towards this end, we also investigate the ability of PDMS-based ring resonators to provide tunable on-chip microfluidic lasers. This idea is explored by looking at two different ring geometries. Very low lasing thresholds are demonstrated.eng
dc.description.bibrefIncludes bibliographical references.eng
dc.format.extentxvi, 155 pageseng
dc.identifier.oclc706833713eng
dc.identifier.urihttps://hdl.handle.net/10355/10248
dc.identifier.urihttps://doi.org/10.32469/10355/10248eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshOptofluidicseng
dc.subject.lcshResonatorseng
dc.subject.lcshDNA -- Methylationeng
dc.subject.lcshMicrofluidicseng
dc.subject.lcshLaserseng
dc.subject.lcshCellular signal transductioneng
dc.titleApplications of the opto-fluidic ring resonator for DNA methylation analysis and microfluidic laser developmenteng
dc.typeThesiseng
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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