dc.contributor.advisor | Fan, Xudong | eng |
dc.contributor.author | Suter, Jonathan Daniel, 1982- | eng |
dc.date.issued | 2010 | eng |
dc.date.submitted | 2010 Fall | eng |
dc.description | Title from PDF of title page (University of Missouri--Columbia, viewed on December 7, 2010). | eng |
dc.description | The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. | eng |
dc.description | Dissertation advisor: Dr. Xudong Fan. | eng |
dc.description | Vita. | eng |
dc.description | Ph. D. University of Missouri--Columbia 2010. | eng |
dc.description.abstract | For several decades, optical ring resonators have played an important role in the field of telecommunications. Within only the past decade however, ring resonators have been applied for the purposes of biological and chemical detection by means of refractometric signal transduction. The advantage of ring resonator-based sensing is that, unlike fluorescence detection, it is label-free and can yield quantitative data. This document presents the fundamental operation of the versatile opto-fluidic ring resonator (OFRR) and explores its applications in DNA methylation analysis. Using affinity assays based on anti-5-methyl cytosine antibodies as well as the methyl binding protein MBD-2, it is shown that the OFRR is a promising tool for biomedical research that can discriminate the extent of DNA methylation. As a part of evolving this and other devices towards a practical laboratory and clinical tool, it is important to consider methods for integrating optical functionality onto a compact chip. Towards this end, we also investigate the ability of PDMS-based ring resonators to provide tunable on-chip microfluidic lasers. This idea is explored by looking at two different ring geometries. Very low lasing thresholds are demonstrated. | eng |
dc.description.bibref | Includes bibliographical references. | eng |
dc.format.extent | xvi, 155 pages | eng |
dc.identifier.oclc | 706833713 | eng |
dc.identifier.uri | https://hdl.handle.net/10355/10248 | |
dc.identifier.uri | https://doi.org/10.32469/10355/10248 | eng |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | OpenAccess. | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. | |
dc.source | Submitted by University of Missouri--Columbia Graduate School. | eng |
dc.subject.lcsh | Optofluidics | eng |
dc.subject.lcsh | Resonators | eng |
dc.subject.lcsh | DNA -- Methylation | eng |
dc.subject.lcsh | Microfluidics | eng |
dc.subject.lcsh | Lasers | eng |
dc.subject.lcsh | Cellular signal transduction | eng |
dc.title | Applications of the opto-fluidic ring resonator for DNA methylation analysis and microfluidic laser development | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Biological engineering (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Doctoral | eng |
thesis.degree.name | Ph. D. | eng |