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dc.contributor.advisorPrather, Randall S.eng
dc.contributor.authorWhitworth, Kristin Marie, 1973-eng
dc.date.issued2010eng
dc.date.submitted2010 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on December 7, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionDissertation advisor: Dr. Randall S. Prather.eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionPh. D. University of Missouri--Columbia 2010.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Animal sciences.eng
dc.description.abstractNuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued development. The first objective was to determine the cloning efficiency in pigs between three activation methods. The second objective was to determine the effects of nuclear transfer on gene expression of blastocyst stage embryos and day 30 placentas and compare that to normal in vivo pregnancies. It was then determined that treatment of reconstructed pig zygotes with the histone deacetylase inhibitor, Scriptaid, returned some of the aberrantly reprogrammed transcripts to normal levels. Although gene expression was similar between the three activation groups, this study identified differentially expressed transcripts between nuclear transfer and normal in vivo embryos and placentas that will help elucidate the reprogramming errors associated with nuclear transfer in pigs.eng
dc.format.extentxvii, 180 pageseng
dc.identifier.oclc706833934eng
dc.identifier.urihttp://hdl.handle.net/10355/10259eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subject.lcshGene expressioneng
dc.subject.lcshSwine -- Embryoseng
dc.subject.lcshProteinseng
dc.subject.lcshCell nuclei -- Transplantationeng
dc.subject.lcshEmbryologyeng
dc.subject.lcshSwine -- Cloningeng
dc.titleDifferential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryoseng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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