[-] Show simple item record

dc.contributor.advisorRay, Bimal K.eng
dc.contributor.authorDhar, Srijitaeng
dc.date.issued2009eng
dc.date.submitted2009 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on March 3, 2011).eng
dc.descriptionVita.eng
dc.descriptionThesis advisor: Bimal K. Ray.eng
dc.descriptionM.S. University of Missouri-Columbia 2009.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] ADAM 12 overexpression is linked to the pathology of diseases including osteoarthritis, cancer, cardiac hypertrophy, Alzheimers and multiple sclerosis. Degradation of fibrous cap and neo chondrogenesis are key events of OA. Several growth promoting agents including transforming growth factor-[beta] (TGF-[beta]) also play determining roles in the function of the cartilage specific cells. The potential link between growth factors and the pathogenesis of OA still remains unresolved. In the present study, we investigated TGF-[beta] mediated transcriptional induction of ADAM 12 in osteoblasts and synoviocytes, two of the resident cells of the joint. CAT assay revealed both positive and negative functioning elements at the proximal and the first exonic region of human ADAM 12 promoter. By using EMSA we showed increased interaction of NF[kappa]B, SAF-1 and Sp1 with the ADAM 12 promoter in TGF-[beta] stimulated cells. At the exonic region we also identified a highly conserved polydinucleotide repeat sequence that acts as a potent basal transcriptional repressor. These results suggest that the cohort action of NF[kappa]B, Sp1 and SAF-1 lead to the overexpression of ADAM 12 under osteoarthritic conditions.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentviii, 92 pageseng
dc.identifier.oclc705358705eng
dc.identifier.urihttps://doi.org/10.32469/10355/10290eng
dc.identifier.urihttps://hdl.handle.net/10355/10290
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campuses of the University of Missouri.eng
dc.subject.lcshMembrane proteins -- Physiological transporteng
dc.subject.lcshOsteoarthritis -- Pathogenesiseng
dc.subject.lcshArticular cartilage -- Metabolismeng
dc.subject.lcshNF-kappa B (DNA-binding protein) -- Metabolismeng
dc.subject.lcshTransforming growth factors-beta -- Metabolismeng
dc.subject.lcshBone cells -- Metabolismeng
dc.subject.lcshSynovial membranes -- Metabolismeng
dc.subject.lcshMetalloproteinases -- Metabolismeng
dc.subject.meshADAM Proteins -- metabolismeng
dc.subject.meshCartilage, Articular -- metabolismeng
dc.subject.meshOsteoarthritis -- pathologyeng
dc.subject.meshTransforming Growth Factor beta -- pharmacologyeng
dc.subject.meshNF-kappa B -- metabolismeng
dc.subject.meshSp1 Transcription Factor -- metabolismeng
dc.subject.meshOsteoblasts -- metabolismeng
dc.subject.meshSynovial Membrane -- metabolismeng
dc.titleTranscriptional regulation of ADAM-12 expression under normal and osteoarthritic conditionseng
dc.typeThesiseng
thesis.degree.disciplineVeterinary pathobiology area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record