[-] Show simple item record

dc.contributor.advisorCannon, John Francis, 1956-eng
dc.contributor.authorAikins, Anthea Anita, 1982-eng
dc.date.issued2010eng
dc.date.submitted2010 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on March 11, 2011).eng
dc.descriptionVita.eng
dc.descriptionThesis advisor: John F. Cannon.eng
dc.description"December 2010"eng
dc.descriptionPh. D. University of Missouri-Columbia 2010.eng
dc.description.abstractGLC7 is an essential gene in Saccharomyces cerevisiae that encodes the catalytic subunit of protein phosphatase-1. Glc7 is conditionally activated by phospho-Glc8, which is phosphorylated by the cyclin dependent kinase, Pho85, associated with Pcl6 and Pcl7 (two of its ten cyclins). Our goal was to determine the input(s) that regulate Pcl6 and Pcl7. This will provide us with valuable information about the conditional activation of Glc7 by phospho-Glc8. We determined Pcl6 to be more stable than Pcl7. We confirmed and discovered that Elongin C (Elc1) stabilizes both Pcl6 and Pcl7. A null mutation of elc1 compromises the in vivo function of Pcl6 and Pcl7 in cell growth and in DNA damage response to 4-nitroquinoline oxide (4-NQO). Elc1 is found in two nucleotide excision repair complexes (NEF4 and Ela1 containing complex). We hypothesized that, Pcl6 and Pcl7 levels are induced by DNA damage and controlled by the Elc1 containing complexes. We discovered that the NEF4 and a non-Elc1 containing complex, NEF2, control the stability of both cyclins. Additionally, DNA damage is an input that controls the conditional activation of Glc7 by phospho-Glc8.eng
dc.description.bibrefIncludes bibliographical references.eng
dc.format.extentxiv, 124 pageseng
dc.identifier.oclc706826985eng
dc.identifier.urihttps://hdl.handle.net/10355/10291
dc.identifier.urihttps://doi.org/10.32469/10355/10291eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subject.lcshSaccharomyces cerevisiae -- Geneticseng
dc.subject.lcshSaccharomyces cerevisiae -- Metabolismeng
dc.subject.lcshPhosphoprotein phosphatases -- Metabolism -- Genetic aspectseng
dc.subject.lcshCyclins -- Metabolism -- Genetic aspectseng
dc.subject.lcshDNA damageeng
dc.subject.lcshDNA repaireng
dc.subject.meshSaccharomyces cerevisiae -- metabolismeng
dc.subject.meshSaccharomyces cerevisiae -- enzymologyeng
dc.subject.meshProtein Phosphatase 1 -- metabolismeng
dc.subject.meshProtein Phosphatase 1 -- geneticseng
dc.subject.meshSaccharomyces cerevisiae Proteins -- metabolismeng
dc.subject.meshSaccharomyces cerevisiae Proteins -- geneticseng
dc.subject.meshCyclins -- metabolismeng
dc.subject.meshCyclins -- geneticseng
dc.subject.meshDNA damageeng
dc.subject.meshDNA repaireng
dc.titleRegulation of Pcl6 and Pcl7 in a Glc7 pathway in Saccharomyces cerevisiaeeng
dc.typeThesiseng
thesis.degree.disciplineMolecular microbiology and immunology (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record