In vitro and in vivo approaches to tumor targeting by phage display
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[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Approximately 30% of breast cancers are linked with the over-expression of ErbB2. Because of its critical role in signal transduction, the progression of breast cancer and its localization on the surface of several types of cancer cells, ErbB2 is a commonly used as a target for the search for protein and peptide ligands that may be useful as therapeutic and diagnostic agents for treating and imaging cancer. In the work described here, I used several commonly used in vitro, ex vivo, and in vivo phage display techniques and binding assays in an attempt to discover such peptides. This work lead to the discovery a phage mutant which interferes with the usage of the phage display libraries of the type used during this research. In addition, I also report the results of a quantitative assessment of a new enzymatically releasable phage construct.
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