Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2007 Summer Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2007 Summer Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Agrobacterium - mediated transformation of sorghum (Sorghum bicolor L.) [abstract]

    Hwang, Kwan Hyun
    Lu, L.
    Wu, Xiaolei R.
    Zhang, Zhanyuan
    Folk, William Robert
    View/Open
    [PDF] Agrobacterium mediated transformation of sorghum.pdf (17.16Kb)
    Date
    2007
    Contributor
    University of Missouri-Columbia. Office of Undergraduate Research
    Format
    Presentation
    Metadata
    [+] Show full item record
    Abstract
    Sorghum variety P898012 was transformed with agrobacterium tumefaciens containing a simple binary vector pSKRS to improve nutritional quality. Immature kernels of sorghum P898012 were harvested after 10 - 12 days pollination. After sterilization, embryos of size 1.0 - 1.6mm were isolated from kernels and immersed in agrobacterium suspension for 5 min, and placed onto co-cultivation medium, scutilla face up for 3 days at 25?. The embryos then transferred onto callus induction medium containing selection agent (Glufosinate 2.5 mg/L) for at least 4 weeks. As soon as herbicide-tolerant calli with somatic embryos developed, calli were transferred onto regeneration medium to develop shoots. At about 3 - 5 weeks, small shoots of height 3 - 5cm were moved into glass tubes containing rooting medium. PCR screening was used for determining transformation events at early stage. The results showed that an average of 2.54 % embryos treated with agrobacterium developed herbicide tolerance tissues on callus induction medium, but all of the condition embryos died of selection. Most of selected tissues developed shoots on regeneration medium, developing roots in rooting medium. After collecting a leaf from each plantlet, 20 DNA samples were purified by REDExtract-N0amptm kit for PCR. After PCR, 17 samples of 20 were positive.
    URI
    http://hdl.handle.net/10355/1121
    Collections
    • 2007 Summer Undergraduate Research and Creative Achievements Forum (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems