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dc.contributor.advisorEmerich, David W. (David William)eng
dc.contributor.authorMitchell, Michael Tayloreng
dc.date.issued2011eng
dc.date.submitted2011 Springeng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on July 22, 2011).eng
dc.descriptionThesis advisor: Dr. David W. Emerich.eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionM.S. University of Missouri--Columbia 2011.eng
dc.description.abstractThe University of Missouri Biochemistry department offers a variety of courses, each designed with a specific purpose and scheduled to avoid overlap. Biochemistry 2002, sophomore lab, is currently an elective course meant to bridge the gap between the introductory Biochemistry 1090 offered to freshmen and Biochemistry 4270, a junior level class. In the past, the course has been burdened by inefficient experiments and lacking in both continuity and structure. My ultimate goal is to improve course coherence by redesigning experiments to increase classroom success rate, cataloging materials to facilitate lab setup, establishing realistic learning objectives, and generating new assignments to be compiled on the university H-drive. The course is subdivided into DNA Cloning and Enzyme Kinetics laboratories. The objective of the first seven weeks is to clone a tobacco acid phosphatase gene, NtPTP1, into the PET28a expression vector. The final four weeks introduces students to a protocol designed to purify the PTP1 protein from an E. coli cell lysate and analyze the activity of the enzyme at several pHs. Upon completion of the course, a solid framework of experiments and concepts has been established, all necessary materials have been catalogued, and a comprehensive list of potential issues has been outlined for future instructors. A new professor will no doubt find it imperative to adjust learning objectives to suit his/her specific teaching goals and personal experience; however, the preexisting foundation should minimize preparation time.eng
dc.format.extentv, 56 pageseng
dc.identifier.urihttp://hdl.handle.net/10355/11497
dc.languageEnglisheng
dc.publisherUniversity of Missouri-Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshUniversity of Missouri--Columbiaeng
dc.subject.lcshBiochemistry -- Study and teaching (Higher)eng
dc.titleImprovement of Biochemistry 2002 through experimental modification and organization of course materialseng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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