Characterizing serotonin in the fruit fly brain [abstract]

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Characterizing serotonin in the fruit fly brain [abstract]

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/1253

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Title: Characterizing serotonin in the fruit fly brain [abstract]
Author: Sable-Smith, Alex; Zars, Troy Daniel
Contributor: University of Missouri-Columbia. Office of Undergraduate Research
Keywords: serotonin
dopamine
Date: 2007
Publisher: University of Missouri--Columbia. Office of Undergraduate Research
Abstract: The biogenic amines serotonin (5-HT) and dopamine (DA) have been implicated in the formation of place memories in Drosophila melanogaster. However, stronger evidence exists in support of 5-HT as the more essential factor in mediating this process. My project focused on characterization of the serotonergic system in fly brains using immunocytochemistry. I probed for 5-HT using a monoclonal antibody and studied its co-localization with neurons expressing GFP driven by either Ddc-Gal4 or DdcThGal80. In an ongoing process, I have so far been able to count 31(+/- 3) 5-HT immunoreactive neurons per hemisphere, 10-15 of which also express GFP. This research works toward a well characterized 5-HT system which can corroborate the various genetic and pharmacological manipulations that are currently used to examine the effects of serotonin on memory formation. I investigated the effects of one of these pharmacological agents, alpha-methyl tryptophan, in addition to the immuno-labeling experiments. After a three-day treatment with the drug, flies showed a lower but not significant decrease in place learning performance as measured using the heat box.
URI: http://hdl.handle.net/10355/1253

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