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dc.contributor.authorSable-Smith, Alexen
dc.contributor.authorZars, Troy Danielen
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researchen
dc.contributor.meetingnameSummer Undergraduate Research and Creative Achievements Forum (2007 : University of Missouri--Columbia)en
dc.date2007en
dc.date.issued2007eng
dc.descriptionAbstract only availableen
dc.description.abstractThe biogenic amines serotonin (5-HT) and dopamine (DA) have been implicated in the formation of place memories in Drosophila melanogaster. However, stronger evidence exists in support of 5-HT as the more essential factor in mediating this process. My project focused on characterization of the serotonergic system in fly brains using immunocytochemistry. I probed for 5-HT using a monoclonal antibody and studied its co-localization with neurons expressing GFP driven by either Ddc-Gal4 or DdcThGal80. In an ongoing process, I have so far been able to count 31(+/- 3) 5-HT immunoreactive neurons per hemisphere, 10-15 of which also express GFP. This research works toward a well characterized 5-HT system which can corroborate the various genetic and pharmacological manipulations that are currently used to examine the effects of serotonin on memory formation. I investigated the effects of one of these pharmacological agents, alpha-methyl tryptophan, in addition to the immuno-labeling experiments. After a three-day treatment with the drug, flies showed a lower but not significant decrease in place learning performance as measured using the heat box.en
dc.identifier.urihttp://hdl.handle.net/10355/1253
dc.languageen_USen
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researchen
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forum
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=en
dc.subjectserotoninen
dc.subjectdopamineen
dc.titleCharacterizing serotonin in the fruit fly brain [abstract]en
dc.typePresentationen


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