Characterizing serotonin in the fruit fly brain [abstract]

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Characterizing serotonin in the fruit fly brain [abstract]

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/1253

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dc.contributor.author Sable-Smith, Alex en
dc.contributor.author Zars, Troy Daniel en
dc.date 2007 en
dc.date.accessioned 2009-04-24T15:44:48Z en
dc.date.available 2009-04-24T15:44:48Z en
dc.date.issued 2007 en
dc.identifier.uri http://hdl.handle.net/10355/1253
dc.description Abstract only available en
dc.description.abstract The biogenic amines serotonin (5-HT) and dopamine (DA) have been implicated in the formation of place memories in Drosophila melanogaster. However, stronger evidence exists in support of 5-HT as the more essential factor in mediating this process. My project focused on characterization of the serotonergic system in fly brains using immunocytochemistry. I probed for 5-HT using a monoclonal antibody and studied its co-localization with neurons expressing GFP driven by either Ddc-Gal4 or DdcThGal80. In an ongoing process, I have so far been able to count 31(+/- 3) 5-HT immunoreactive neurons per hemisphere, 10-15 of which also express GFP. This research works toward a well characterized 5-HT system which can corroborate the various genetic and pharmacological manipulations that are currently used to examine the effects of serotonin on memory formation. I investigated the effects of one of these pharmacological agents, alpha-methyl tryptophan, in addition to the immuno-labeling experiments. After a three-day treatment with the drug, flies showed a lower but not significant decrease in place learning performance as measured using the heat box. en
dc.language en_US en
dc.language.iso en_US en
dc.publisher University of Missouri--Columbia. Office of Undergraduate Research en
dc.source.uri http://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid= en
dc.subject serotonin en
dc.subject dopamine en
dc.title Characterizing serotonin in the fruit fly brain [abstract] en
dc.type Presentation en
dc.contributor.meetingname Summer Undergraduate Research and Creative Achievements Forum (2007 : University of Missouri--Columbia) en
dc.contributor.corporatename University of Missouri-Columbia. Office of Undergraduate Research en
dc.relation.ispartofcommunity University of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forum


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