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dc.contributor.authorUnverferth, Julieeng
dc.contributor.authorLo, Shih-Ching, 1979-eng
dc.contributor.authorHannink, Mark, 1958-eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameSummer Undergraduate Research and Creative Achievements Forum (2007 : University of Missouri--Columbia)eng
dc.date2007eng
dc.date.issued2007eng
dc.descriptionAbstract only availableeng
dc.description.abstractPGAM5 is a member of the phosphoglycerate mutase family. It has been identified as a substrate for BTB-Kelch protein Keap1, a substrate adaptor for a cullin-based ubiquitin ligase. Keap1 plays an important role in cellular redox homeostasis through negative regulation of transcription factor Nrf2. Recent evidence suggests that PGAM5, Keap1 and Nrf2 may form a ternary complex that contributes to the regulation of anti-oxidant gene expression. In order to understand the role of PGAM5, we will identify its interacting partners through yeast-two hybrid screening. Fusions of the binding domain and four constructs of PGAM5 were cloned and subsequently transformed into yeast cells. These fusions were tested to ensure that they do not autonomously activate reporter genes, which suggests protein interaction. The yeast containing the fusions will be mated with a library of proteins fused to the activation domain. The progeny that activate reporter genes will suggest novel interacting protein partners for PGAM5.eng
dc.identifier.urihttp://hdl.handle.net/10355/1285eng
dc.languageen_USeng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=eng
dc.subjectphosphoglycerate mutase familyeng
dc.subjectcellular redox homeostasiseng
dc.titleYeast two-hybrid screen of PGAM5 [abstract]eng
dc.typePresentationeng


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