Sensor Organosilicate Nanoparticles for Diagnostic Devices and Environmental Devices
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This invention reports a novel technique for the rapid and cost-efficient synthesis of organosilicate nanoparticles (OSNPs) that have been successfully applied as individual building blocks for various applications. Through our patented technology of novel bottom up fabrication technique, these nanoparticles have been used to fabricate highly porous transparent films. Optically smooth hydrophobic films with low refractive indices (as low as 1.048) and high surface areas (as high as 1325 m2/g) can be achieved on large area substrates. These films have been studied and used for a solid phase microextraction (SPME) membrane for identification and quantification of polychlorobiphenyls (PCBs) for replacement of polydimethylsiloxane (PDMS) membranes which are surrently used to detect PCBs in underground water. Furthermore, doping these nanoparticles with fluorescent dyes results in highly fluorescent, biocompatible and water soluble nanoparticles with demonstrated long term photostability and with surface groups that can be readily used to attach various biological moieties. Fluorescent intensity of dye-doped OSNPs (22.4 ± 5.3 nm) is shown to be 200 times brighter than the constituent dyes. These unique materials can be readily interfaced with existing immunoassays in the form of inexpensive dip-stick assays for the novel diagnostic strips for point of care applications. Our preliminary evaluation of these films in combination with dye-doped OSNPs for construction of diagnostic immunoassays gave ~180 fold enhancement in fluorescence signal enhancement compared to traditional (microscope glass slide and fluorescent dye molecules) based assays. Potential Areas of Applications: Diagnosis of CNS diseases, Monitor the efficacy of treatment of CNS disease, Estimate the degree of CNS tumor growth, Measure progression of clinical and preclinical pathologies in drug trials, Surgery guidance. Patent Status: U.S. Patent 7,907,809. Inventor(s): Shubhra Gangopadhyay, Keshab Gangopadhyay, Venumadhav Korampally, Sangho Bok. This presentation was an elevator pitch at the Missouri Technology Expo 2011.