Detecting microRNA (miRNA) using nanopore single molecule detector

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Detecting microRNA (miRNA) using nanopore single molecule detector

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[-] show simple item record Gu, Li-Qun 2012-02-24T19:14:49Z 2012-02-24T19:14:49Z 2011-09 eng
dc.description Information used in this entry was gathered at en_US
dc.description.abstract MicroRNAs (miRNAs) are a class of short (~18-24-nt) non-coding RNAs molecules that regulate gene expression at the post- transcriptional level. Aberrant expression of miRNAs has been found in all types of tumors. Most notably, specific miRNAs are released from the primary tumor into blood circulation, making the detection of circulating miRNAs a powerful tool for noninvasive cancer detection, diagnosis, staging, and monitoring. We developed a robust nanopore sensor that selectively detects single molecules of circulating miRNAs derived from primary cancer. The nanopore is a fabricated 2-nm molecular pore. Such a tiny pore can generate a signature current signal when a miRNA molecule is specifically captured in it. These signals function as fingerprints that enable us to identify a specific miRNA and quantify its concentration. The prototype has demonstrated the capability to discriminate single nucleotide difference between miRNAs. In clinical tests, we have differentiated miRNA levels in blood between lung cancer patients and healthy people. Due to the label-free single molecule detection without nucleic acids amplification, the nanopore sensor is higher selective, precise and accurate over the gold standard RT-PCR and microarray. The nanopore array in development would achieve high throughput capability for detecting miRNA profile. Potential Areas of Applications: Detection of any nucleic acids fragments, including all microRNAs and pathogenic DNAs or RNAs, Study of nucleic acid folding/unfolding reactions, Study of miRNA mechanism and principle of regulation Study single nucleotide polymorphisms (SNPs), Study of oligonucleotide-protein and oligonucleotide-drug interactions for drug discovery and development, Biomarker characterization, Diagnostics, prognostics and theranostics. Patent Status: Patent pending. Inventor(s): Li-Qun Gu, Yong Wang. en_US
dc.format.extent 7:46 minutes
dc.language.iso en_US en_US
dc.relation.ispartof Videos (Missouri Technology Expo 2011)
dc.subject MicroRNA en_US
dc.subject noninvasive cancer detection en_US
dc.subject cancer research en_US
dc.subject nanopore en_US
dc.subject nucleic acid folding en_US
dc.subject single nucleotide polymorphism en_US
dc.subject.lcsh Diagnosis, Noninvasive
dc.subject.lcsh Cancer -- Diagnosis
dc.subject.lcsh Non-coding RNA
dc.title Detecting microRNA (miRNA) using nanopore single molecule detector en_US
dc.type Video en_US
dc.contributor.meetingname Missouri Technology Expo (2011 : Columbia, Mo.)
dc.relation.ispartofcommunity University of Missouri-Columbia. Missouri Technology Expo. Missouri Technology Expo 2011

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