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dc.contributor.advisorBilyeu, Kristin D.eng
dc.contributor.advisorShannon, J. Grovereng
dc.contributor.authorLenis, Julian Marioeng
dc.date.issued2011eng
dc.date.submitted2011 Summereng
dc.description"July 2011"eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on May 17, 2012).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionDissertation advisors: Dr. Kristin D. Bilyeu and Dr. J. Grover Shannoneng
dc.descriptionVita.eng
dc.description.abstractSoybean seeds, valuable feed and food resources, have high protein and oil contents, with a fairly balanced amino acid profile and abundant essential fatty acids (FA). Linolenic acid (18:3), an essential [omega]-3 type of FA, makes up to 8% of typical soybean seed oil. In humans, [omega]-3 FAs play a crucial role in brain function as well as normal growth and development. Glycine soja typically has twice as much 18:3 as soybean. However, very little is known about the genetic regulation of high 18:3 in G. soja. The role of microsomal FAD2 and FAD3 as well as DGAT, PDAT and PDCT genes in 18:3 accumulation in G. soja seeds was investigated. Structural and functional analyses of the genes were carried out. The relative greater contribution of PDAT in the final acylation step of triacylglycerol synthesis might be partially responsible for the higher 18:3 content in G. soja seeds. Also, the continuous distribution of phenotypes and large environmental influence indicate 18:3 is multigenic. Breeding for increased 18:3 seed content with G. soja as the donor has an impact on other traits of importance. In addition, 18:3 in seeds is usually oxidized by lipoxygenases, resulting in the development of undesirable flavor and aroma. Soybean seeds contain three lipoxygenases encoded by Lox1, Lox2 and Lox3. Null alleles have been identified. Previous studies determined that a missense mutation rendered Lox2 inactive. The basis of either lox1 or lox3 mutation was not known. The genetic basis of lox1 and lox3 mutant alleles was determined, and molecular markers that allow efficient selection for lox1, lox2 and lox3 mutant alleles were developed.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxv, 275 pageseng
dc.identifier.oclc872561135eng
dc.identifier.urihttps://doi.org/10.32469/10355/14213eng
dc.identifier.urihttps://hdl.handle.net/10355/14213
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertations.eng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subjectfatty acideng
dc.subjectoil compositioneng
dc.subjectlipoxygenaseeng
dc.subjectlinolenic acideng
dc.titleGenetics of soybean seed lipoxygenases and linolenic acid content in seeds of the soybean wild ancestoreng
dc.typeThesiseng
thesis.degree.disciplinePlant, insect and microbial sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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