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dc.contributor.advisorIslam, Naz E.eng
dc.contributor.advisorGanjam, V.K.eng
dc.contributor.authorAmruthambaeng
dc.date.issued2011eng
dc.date.submitted2011 Summereng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on May 24, 2012).eng
dc.descriptionThe entire thesis text is included in the research.pf file; the official abstract appears in the short.pf file; a non-technical public abstract appears in the public.pf file.eng
dc.descriptionThesis advisors: Dr. Naz Islam, Dr. V.K. Ganjameng
dc.descriptionIncludes bibliographical references.eng
dc.description"July 2011"eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Effects of nanosecond pulsed electric fields on the human breast cancer cell line MCF-7 have been investigated for changes in cell number, DNA concentration, DNA synthesis rate, and 11[lowercase beta]HSD2 enzyme activity. Pulse trains of ten 32.5 kV/cm, 60 ns pulses, generated by means of a specific pulse-forming network, were applied to a pair of electrodes in electroporation cuvettes through a spark-gap switch. The experimental results show a 20% increase in cell death for treated cells, one hour after pulsing and before reculturing, with a 60% decrease in DNA concentration. Our results also indicate a 19% inhibition rate of the 11[lowercase beta]HSD2 enzyme activity in treated MCF-7 cells seven days following exposure. This spares the endogenous glucocorticoids from both degradation (apoptosis) and inactivation, a fact that is herein demonstrated for the first time.eng
dc.format.extentvii, 61 pageseng
dc.identifier.urihttp://hdl.handle.net/10355/14366
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess to files is limited to the University of Missouri--Columbia.eng
dc.subjectbreast cancereng
dc.subjectcancer therapyeng
dc.subjectDNA synthesiseng
dc.subjectpulsed electric fieldeng
dc.titleEffects of nano-second pulsed electric fields on human breast cancer cell line-MCF7eng
dc.typeThesiseng
thesis.degree.disciplineElectrical engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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