Soil microbial contribution to greenhouse gas efflux from a secondary forest in central Missouri

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Soil microbial contribution to greenhouse gas efflux from a secondary forest in central Missouri

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/14496

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dc.contributor.advisor Kremer, R. J. (Robert J.) en_US
dc.contributor.advisor Goyne, Keith William en_US
dc.contributor.author Hoilett, Nigel
dc.date.accessioned 2012-06-04T16:25:16Z
dc.date.available 2012-12-16T12:15:07Z
dc.date.issued 2011
dc.date.submitted 2011 Fall en_US
dc.identifier.other HoilettN-121911-D121
dc.identifier.uri http://hdl.handle.net/10355/14496
dc.description Title from PDF of title page (University of Missouri--Columbia, viewed on June 4, 2012). en_US
dc.description The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. en_US
dc.description Dissertation advisors: Dr. Robert Kremer, Dr. Keith W. Goyne en_US
dc.description Vita. en_US
dc.description Includes bibliographical references. en_US
dc.description Ph. D. University of Missouri--Columbia 2011. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Soil, environmental, and atmospheric sciences. en_US
dc.description "December 2011" en_US
dc.description.abstract Greenhouse gas emissions (GHG) vary with the interactions among physical, chemical, and biological characteristics of soil and microclimate. Soil microorganisms are involved in almost all soil processes, and are also involved in GHG dynamics between the soil and atmosphere. The objective of this study was to examine the relationship between GHG efflux and soil microbial community and activity across a forested landscape. Soil samples were incubated at different temperature and moisture over a 30 days period. Microbial activity and diversity were assessed using enzyme activity, phospholipid lipid fatty acid profiles (PLFA), and soil microbial diversity, based on polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and real-time PCR analysis. Our research revealed temporal differences in microbial population and GHG efflux, indicating that time of year when samples were collected is an important factor. Temperature and moisture significantly affected soil microbial population and GHG efflux. In addition, correlation between GHG and measured biological properties and GHG and soil temperature and moisture in the incubated soils implied that microbial properties as well as soil temperature and moisture may affect GHG efflux from these forest soils. However, the low correlation coefficient (r values) and the lack of correlation within some sampling periods indicated that the relationship among soil microorganisms, soil conditions and GHG efflux is highly complex and cannot be fully explained by direct correlations among the measured properties and GHG efflux. en_US
dc.format.extent xv, 168 pages en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject greenhouse gases en_US
dc.subject soil microorganisms en_US
dc.subject phospholipid fatty acid en_US
dc.subject denaturing gradient gel electrophoresis en_US
dc.title Soil microbial contribution to greenhouse gas efflux from a secondary forest in central Missouri en_US
dc.type Thesis en_US
thesis.degree.discipline Soil, environmental and atmospheric sciences en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name Ph. D. en_US
thesis.degree.level Doctoral en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2011 Dissertations
dc.relation.ispartofcollection 2011 Freely available dissertations (MU)


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