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dc.contributor.advisorKremer, R. J. (Robert J.)en_US
dc.contributor.advisorGoyne, Keith Williamen_US
dc.contributor.authorHoilett, Nigel
dc.date.issued2011
dc.date.submitted2011 Fallen_US
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 4, 2012).en_US
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.en_US
dc.descriptionDissertation advisors: Dr. Robert Kremer, Dr. Keith W. Goyneen_US
dc.descriptionVita.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionPh. D. University of Missouri--Columbia 2011.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Soil, environmental, and atmospheric sciences.en_US
dc.description"December 2011"en_US
dc.description.abstractGreenhouse gas emissions (GHG) vary with the interactions among physical, chemical, and biological characteristics of soil and microclimate. Soil microorganisms are involved in almost all soil processes, and are also involved in GHG dynamics between the soil and atmosphere. The objective of this study was to examine the relationship between GHG efflux and soil microbial community and activity across a forested landscape. Soil samples were incubated at different temperature and moisture over a 30 days period. Microbial activity and diversity were assessed using enzyme activity, phospholipid lipid fatty acid profiles (PLFA), and soil microbial diversity, based on polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and real-time PCR analysis. Our research revealed temporal differences in microbial population and GHG efflux, indicating that time of year when samples were collected is an important factor. Temperature and moisture significantly affected soil microbial population and GHG efflux. In addition, correlation between GHG and measured biological properties and GHG and soil temperature and moisture in the incubated soils implied that microbial properties as well as soil temperature and moisture may affect GHG efflux from these forest soils. However, the low correlation coefficient (r values) and the lack of correlation within some sampling periods indicated that the relationship among soil microorganisms, soil conditions and GHG efflux is highly complex and cannot be fully explained by direct correlations among the measured properties and GHG efflux.en_US
dc.format.extentxv, 168 pagesen_US
dc.identifier.otherHoilettN-121911-D121
dc.identifier.urihttp://hdl.handle.net/10355/14496
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2011 Freely available dissertations (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2011 Dissertations
dc.subjectgreenhouse gasesen_US
dc.subjectsoil microorganismsen_US
dc.subjectphospholipid fatty aciden_US
dc.subjectdenaturing gradient gel electrophoresisen_US
dc.titleSoil microbial contribution to greenhouse gas efflux from a secondary forest in central Missourien_US
dc.typeThesisen_US
thesis.degree.disciplineSoil, environmental and atmospheric sciencesen_US
thesis.degree.disciplineSoil, environmental and atmospheric scienceseng
thesis.degree.grantorUniversity of Missouri--Columbiaen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePh. D.en_US


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